Correlative Study Of Granulocyte Macrophage Colony-stimulating Factor On Gastric Cancer Proliferation,Migration And Drug Resistance

Objective Granulocyte macrophage colony stimulating factor(GM-CSF)is a hematopoietic cell proliferation and differentiation stimulating factor.More and more studies have found that tumor cells can autocrine GM-CSF,which will affect the proliferation,invasiveness and drug resistance of tumor cells.However,there are relatively few studies on the progress and resistance of GM-CSF to gastric cancer,and more in-depth research on related mechanisms is unclear.In this experiment,by examining the effect of GM-CSF on the proliferation and migration of gastric cancer cells and the effect on the apoptosis and cycle of gastric cancer cells induced by fluorouracil(5-FU),this study is to investigate the proliferation,migration and resistance of GM-CSF to gastric cancer cells The sexual impact provides a theoretical basis for the development of antitumor drugs targeting GM-CSF.Methods 1.The gastric cancer SGC7901 cells were cultured in vitro,and the effect of GM-CSF on the proliferation of gastric cancer was detected by plate cloning experiment.Cell scratch test was used to observe the effect of GM-CSF on the migration of gastric cancer cells.2.The MTT method was used to detect the half-lethal dose of 5-FU on gastric cancer cells.GM-CSF was added to the 5-FU-treated gastric cancer cells.3.Annexin V-FITC / PI flow cytometry was used to detect apoptosis,and PI flow cytometry was used to detect cell cycle.4.Western blotting was used to detect the expression of apoptosis-related proteins Bax and Bcl-2.Results1.GM-CSF can not only promote the proliferation of gastric cancer cells,but also promote the migration of gastric cancer cells.2.The half lethal dose of 5-FU to gastric cancer cells is(16 ± 0.4)mg / L.5-FU has a significant inhibitory effect on gastric cancer cell activity,and GM-CSF can effectively reduce the inhibitory effect of 5-FU.3.Annexin V-FITC / PI flow cytometry showed that 5-FU can significantly induce gastric cancer cell apoptosis.After GM-CSF was added,5-FU-induced gastric cancer apoptosis was resisted.4.Flow cycle results showed that 5-FU and GM-CSF can cause gastric cancer cell cycle arrest,and the two have antagonistic effects.5.Western blotting showed that the expression of apoptosis-related proteins showed that the ratio of Bax / Bcl-2 induced by 5-FU in gastric cancer cells was significantly increased.After GM-CSF was added,5-FU-induced apoptosis was resisted and Bax / Bcl-2 The upward trend of the ratio is suppressed.Conclusion In this experiment,GM-CSF can promote the proliferation and migration of gastric cancer cells,and effectively inhibit the 5-FU-induced apoptosis and cycle arrest of gastric cancer cells,which plays an important role in enhancing the chemoresistance of gastric cancer cells.GM-CSF may promote the drug resistance of gastric cancer cells by inhibiting the expression of apoptosis related protein Bax.