| Objective By detecting the key inflammatory factor TLR-2 of the Toll-like receptor signaling pathway and the downstream related inflammatory factors tumor necrosis factor ?(TNF-?),interleukin 1(IL-1),and matrix metalloproteinase 9(MMP-9)Expression in articular cartilage under weight-bearing and non-weight-bearing areas in patients with primary knee osteoarthritis with varus deformity,and to explore its association with the onset of knee osteoarthritis(KOA)and Whether the progression of the disease is relevant.Methods Forty patients(8 males and 32 females)with primary knee osteoarthritis and varus deformity who underwent artificial total knee arthroplasty at the General Hospital of ××× Medical University from January 2018 to January 2019.Collect the basic medical records of the patients before,during and after the operation,collect the patients' epiphyseal joints during the operation,and take the specimens as clinical samples.The articular cartilage with severe abrasion in the weight-bearing area of the medial femoral condyle was used as the experimental group.The normal articular cartilage in the upper non-weight-bearing area was used as a control group,and decalcified paraffin sections were routinely prepared.Hematoxylin and eosin staining(HE)and Safranin O staining were used to observe negative and non-negative recombination.Histopathological morphology;observation and comparison of ultrastructural characteristics of experimental group and control group by scanning electron microscope;detection of TLR-2,TNF-?,IL-1,MMP-9 in experimental group and control group by immunohistochemical staining The expression of the positive cells was observed and recorded in the articular cartilage in a random field of view with a 20 x light microscope,and the statistics were counted.The results were statistically analyzed using the SPSS22.0 statistical analysis software.Results(1)HE staining: Compared with the control group,the articular cartilage in the load-bearing area of the experimental group has a reduced number of articular chondrocytes and uneven matrix staining.(2)Safranin O staining: The articular cartilage staining in the load-bearing area of the experimental group showed a change in the shape of chondrocytes,the subchondral bone was exposed,the tide line disappeared,and the matrix could have deep or decolorized staining.The cells and cell matrix were stained,arranged and distributed uniformly.(3)Scanning electron microscope observation results: The structure of the articular cartilage surface in the experimental group was unclear,and a large number of pores of different sizes were seen.Collagen fibers were not formed and normal chondrocytes were rare.Bundle and neatly arranged,normal chondrocytes can be seen.(4)Immunohistochemical staining: TLR-2,TNF-?,IL-1,and MMP-9 were all expressed in the experimental group and the control group.The TLR-2 positive cell rate in the experimental group was the highest(0.56 ± 0.03).),Followed by TNF-?(0.49 ± 0.04),MMP-9(0.41 ± 0.03)and IL-1(0.40 ± 0.02).In the control group,TLR-2 was also expressed in non-weight-bearing regions and higher than TNF-?,IL-1,and MMP-9,which were(0.30 ± 0.02,0.18 ± 0.02,0.21 ± 0.01,0.16 ± 0.02),and passed SPSS22.0 Statistical analysis The expressions of inflammatory factors in each cell of the experimental group and the control group were statistically different(p <0.05).Conclusion TLR-2,TNF-?,IL-1,and MMP-9 were all expressed in articular chondrocytes of patients with knee osteoarthritis with varus deformity,and the expression of the articular chondrocytes in the weight-bearing area of the experimental group was higher than that of the cells.The expression of articular chondrocytes in the non-weight-bearing area of the control group suggested that the Toll-like receptor signal transduction pathway and its downstream related cellular inflammatory factors may participate in and mediate the onset and progression of knee osteoarthritis. |
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