Effects Of Yishen Capsule On The Expression Of LC3? And P62 In Renal Tissue Of Diabetic Nephropathy Rats

Objective:To explore the effect of Yishen Capsule on the expression of LC3? and p62 in renal tissue of diabetic nephropathy rats.Methods:Forty male SD rats were randomly divided into four groups,namely the normal group,the diabetic nephropathy group,the Yishen Capsule group and the resveratrol group,with10 rats in each group.The Yishen Capsule group were given Yishen Capsule,at a dose of0.625g/kg�d for 8 weeks.And the resveratrol group were given resveratrol,at a dose of30mg/kg�d for 8 weeks.The normal group and the diabetic nephropathy group were given the same amount of normal saline for 8 weeks.Observe the general state changes of the rats,and measure the body weight,blood glucose and 24-hour urine protein of the four groups at the end of 0,4 and 8 weeks.At the end of 8 weeks,blood was taken from the abdominal aorta of the four groups to measure the related biochemical indexes such as cholesterol(TC),triglyceride(TG),serum creatinine(Scr)and blood urea nitrogen(BUN).The pathological changes of renal tissue were detected by HE and PAS staining.The expression of autophagy related protein LC3? and ubiquitin-binding protein p62(p62/SQSTMI)were detected by immunohistochemistry.Results:(1)Compared with the normal group,the weight of rats in diabetic nephropathy group decreased significantly at the end of 8 weeks(P<0.05).Compared with the diabeticnephropathy group,the weight of rats in Yishen Capsule group and resveratrol group increased at the end of 8 weeks(P<0.05).Compared with the normal group,the blood glucose of diabetic nephropathy group increased significantly at the end of 0,4 and 8weeks(P<0.05).Compared with the diabetic nephropathy group,there was no significant difference in blood glucose between Yishen Capsule group and resveratrol group(P>0.05).(2)Compared with the normal group,the quantitative values of serum creatinine,blood urea nitrogen,cholesterol,triglyceride and 24-hour urine protein in diabetic nephropathy group were increased(P<0.05).Compared with the diabetic nephropathy group,the quantitative values of serum creatinine,blood urea nitrogen,cholesterol,triglyceride and 24-hour urine protein in Yishen Capsule group and resveratrol group were decreased(P<0.05).(3)Compared with the normal group,the mesangial matrix hyperplasia of diabetic nephropathy group was more obvious;the renal tubules narrowed,the epithelial cells of renal tubular were edematous,the renal tubules showed vacuolar degeneration,the expression of autophagy related protein LC3? was decreased(P<0.05),the expression of autophagy related protein p62 was increased(P<0.05).Compared with the diabetic nephropathy group,the renal pathological changes of rats in Yishen Capsule group and resveratrol group were significantly reduced,the mesangial matrix hyperplasia and the edema of renal tubular epithelial cells were all improved at different degree,but vacuolar degeneration were still found in some renal tubules,the expression of LC3? was increased(P<0.05),and the expression of p62 was decreased(P<0.05).Conclusion:Yishen Capsule may play a protective role in diabetic nephropathy by improving the autophagy of renal tissue in diabetic nephropathy rats.