Preliminary Analysis Of Intestinal Microflora In Patients With Different Severity Of OSAHS

Objective:In this study,16 S rRNA next-generation sequencing technology is used to perform high-throughput sequencing on feces of OSAHS patients with different severity.Bioinformatics,statistics and other methods are used to analyze the diversity,abundance and structure of intestinal flora of patients with different severity OSAHS Composition and other characteristics,and preliminary explore its potential role in the development of OSAHS.Methods:The 100 selected OSAHS patients were all from the Sleep Medicine Center of the Second Hospital of Shanxi Medical University.In addition,27 healthy volunteers were selected as controls in the Health Checkup Center of the hospital.All enrolled patients underwent PSG monitoring and collected indicators such as AHI,SpO2 min,SpO2mean and Tmax.According to the latest international classification of sleep disorders(3rd edition)diagnostic criteria and the presence or absence of comorbidities,the patients were divided into mild,moderate and severe simple OSAS and severe OSAHS with complication group.BMI).All enrolled persons collected stool samples for 16 S rRNA high-throughput sequencing,and analyzed the data using analysis software such as Chellime2 and SPSS 22.0.Results:1.Alpha and Beta diversity analysis of all sample flora showed that there was no significant difference in the overall abundance,diversity and structural composition of intestinal flora between the groups,and the results were not statistically significant(P>0.05);2.Species composition analysis,each group of dominant bacteria mainly consists of Firmicutes,Bacteroidetes,Proteobacteria,Actinobacteria,Fusobacteria and Verrucomicrobia.At the door level,the relative abundance of Bacteroidetes in each OSAHS group was lower than that in the normal group;3.Generic level analysis showed that the relative abundance of Bacteroides and Faecalibacterium showed a decreasing trend with the aggravation of OSAHS.The relative abundance of Bifidobacterium,Blautia and Ruminococcus showed an increasing trend with the aggravation of OSAHS;4.Compared with the simple severe OSAHS group,the relative abundance of Roseburia in the severe OSAHS group with complications is lower,and the relative abundance of Shigella is higher;5.Lefse analysis showed that compared with the severe OSAHS group,the abundant expression of Enterobacteriales and Shigella was higher in the severe OSAHS group with complications(P <0.05);6.Redundancy analysis of RDA shows that,except that BMI is positively correlated with the overall abundance of intestinal flora,other indicators such as AHI,SpO2 min,SpO2mean and Tmax have no significant correlation with the overall abundance of intestinal flora(P> 0.05)7.Spearman correlation analysis showed that the abundances of Shigella and Blautia were negatively correlated with BMI,and the abundances of Actinomycetospora and Akkermansia were positively correlated with BMI;the abundances of Thermus,Sediminibacterium,Ralstonia,Pelomonas,Blautia,Anoxybacillus and Anaerofustis were shown to be AHI and Tmax Negative correlation,positive correlation with SpO2 min,P values <0.05.Conclusion:1.OSAHS patients’ intestinal flora structure has changed to a certain extent.The gate level found that the ratio of Firmicutes / Bacteroidetes(F / B)increased to varying degrees,suggesting that there is an microecological intestinal imbalance in OSAHS patients;2.With the aggravation of OSAHS,the relative abundance of short-chain fatty acid-producing probiotics such as Faecalibacterium and Roseburia showed an overall downward trend,while the relative abundance of pathogenic bacteria Shigella showed an upward trend,suggesting a disorder of intestinal flora May be related to the occurrence and development of the disease.Provides new ideas for the exploration of the pathogenesis of OSAHS,and also provides the basis for the application of new treatment methods such as probiotic intervention in the future;3.The relative abundance of probiotics,such as Bifidobacterium and Ruminococcus,increases with the severity of OSAHS,which may be related to the influence of daily dietary habits and probiotic interventions,or the change of bacterial flora with environmental adaptability;4.Some microbial species such as Enterobacteriales and Shigella can be used as potential biomarker species to distinguish severe OSAHS patients from complications,and provide a theoretical basis for disease risk assessment of OSAHS based on intestinal microbes;5.Part of the genus shows a significant correlation with clinical sleep monitoring indicators,which provide the possibility of predicting and evaluating disease risk based on clinical indicators combined with intestinal flora.