Expression Of Wnt/?-catenin Signaling Pathway Antagonist Genes SFRP1, WIF1, RUNX3 And Relationship With The Pathogenesis Of Oropharyngeal Cancer

With the changes in dietary structure,living habits and environment in China,the incidence rate of oropharyngeal cancer is rising year by year.Development from the normal mucosal dysplasia of oropharyngeal cancer is a multiple stages and polygenes involved process.It has been found in recent years that the aberrant activation of Wnt/?-catenin signaling pathway is closely related to oral tumors.The dysregulation of Wnt/?-catenin signaling elements is involved in aberrant proliferation,migration and invasion of cells,which leads to tumor.Wnt antagonists include the secreted frizzled-related protein(SFRP)family,Wnt inhibiting factor-1(WIF-1)and RUNX families.Researches has proved that antagonist functions as an oncogene in different human carcinogenesis.Study on the expression pattern,biological function and molecular mechanism of Wnt antagonists in oropharyngeal cancer will contribute to understanding the pathogenesis,therapeutic strategy and prognosis of this disease.The aim of our study was to investigate the role and function of SFRP1,WIF1,RUNX3 on oropharyngeal carcinoma,to analyze its relationship with clinicopathology and prognosis,and to determine the mechanism of tumorigenesis.By knocking down of SFRP1,WIF1,RUNX3,the abilities of proliferation and invasion of CAL27 cells were impaired,and the Wnt/?-catenin signaling pathway was activated.They may be a potential target for diagnosis,treatment and prognosis evaluation of oropharyngeal carcinoma.