~1H NMR Based Metabolomics Approach To Evaluate Preventive And Therapeutic Effects Of Gastrodia Elata Blume On Chronic Atrophic Gastritis

Chronic atrophic gastritis(CAG)is one of the most common digestive system diseases worldwide which defined by WHO as initial step of cancer.Gastrodia elata Blume(GEB)is a traditional herbal with multiple pharmacological activities which was widely used in Asian countries.The aim of this study was to investigate the effects of autoimmune-induced chronic atrophic gastritis on metabolites in rats and to evaluate the preventive and therapeutic effects of Gastrodia elata on chronic atrophic gastritis.In this study,rats were divided into six groups: blank control group(CON,saline),model group(MCG,saline),prophylactic administration group(PGE,0.4g/kg),high dose group(HE,0.4g/kg),low dose group(LGE,0.1g/kg)and positive drug group(DOM,1mg/kg).For the first time,we used 1H NMR-based metabonomics with histopathological examination to analyze the stomachs of rats.The biochemical indexes of MDA,SOD,GSH,NO and XOD were measured.The results showed that GEB had a significant therapeutic effect on chronic atrophic gastritis at the dose of 0.4g/kg.Gastrodia elata Blume could apparently ameliorate the damaged gastric glands and the biochemical parameters,enhance gastric acid secretion,and significantly relieve the inflammation of the stomach.Orthogonal signal correction-partial least squaresdiscriminant analysis(OSC-PLS-DA)of NMR profiles and correlation network analysis revealed that Gastrodia elata Blume could effectively treat CAG via regulating energy and purine metabolisms,and by anti-oxidation and anti-inflammation effects.In the experiment of evaluating the pharmacodynamics of different polar parts,Gastrodia elata was divided into four polar parts: water phase,n-butanol phase,ethyl acetate phase and petroleum ether phase.The therapeutic effect of different polar parts on chronic atrophic gastritis was evaluated at a dose of 0.4 g/kg.Metabonomic analysis showed that the extracts from water phase and n-butanol phase of Gastrodia elata had better pharmacodynamic effect.Therefore,we speculated that the antioxidant and anti-inflammatory components of Gastrodia elata were concentrated in the large polar part.