| Angelica is an authentic medicinal material in Gansu Province,with large output,and volatile oil is one of its main biologically active ingredients.Angelica stems and leaves are discarded at the early stage of harvest,resulting in waste of resources.Studies have shown that angelica stems and leaves also contain volatile oil.The research on the antioxidant and antibacterial aspects of the volatile oil of traditional Chinese medicine is relatively mature,but there are few reports on the antioxidant and antibacterial activities of the volatile oil of Angelica sinensis and its stems and leaves.The development and utilization of Angelica sinensis and its stems and leaves resources are of great significance.In this experiment,the roots,stems and leaves of Angelicasinensis were taken as the research objects,and the volatile oil from the roots,stems and leaves of Angelicasinensis was extracted by steam distillation;carried out the method ofgas chromatography integrated with mass spectrometry(GC-MS)to establish 10 batches of volatile oil fingerprints of roots,stems and leaves of Angelicasinensis;The results showed that the volatile oil content of Angelicasinensis roots was significantly higher than that of stems and leaves(P<0.01),the content of volatile oil from root was about twice that from stems and leaves of Angelica sinensis.Fifty-one compounds were identified in the common pattern of 10 batches of volatile oil from stems and leaves of Angelicasinensis,23 compounds were identified in roots volatile oil,and 6 compounds in roots,stems and leaves volatile oil,the content of of(1R)-?-pinene,(1s)-?-pinene,butenylphthalein and Z-ligustilide was significantly different(P<0.01).Carried out the method of scavenging1,1-diphenyl-2-trinitrophenyl hydrazine(DPPH),2,2-diazo-bis(3-ethyl-benzothiazole-6-sulfonic acid)diammonium salt(ABTS)free radical for determination of antioxidant activity of volatile oil fromroots,stems and leaves of AngelicaSinensis.The results showed that different batches of volatile oil from Angelicasinensisroots,stems and leaves had different degrees of scavenging effect on DPPH and ABTS,the half maximal inhibitory concentration(IC50)value of the DPPH and ABTS free radicals scavenged by the volatile oil fromAngelicasinensisstems and leaves was significantly less than that of Angelicasinensisstems and leaves(P<0.01).The antibacterial activity of the volatile oil fromroots,stems and leaves of AngelicaSinensis was evaluated by the diameter of the inhibition zone,the minimum inhibitory concentration(MIC)and the minimum bactericidal concentration(MBC).The results showed that the diameters of inhibition zone in the volatile oil from roots,stems and leaves of Angelicasinensis in different batches were between 6.5 mm-12.11 mm,moreover,the diameters of the inhibition zone of volatile oil fromAngelica roots,stems and leaves acting on gram-negative bacteriumwere larger than those of gram-positive bacterium.The MIC of the three gram-positive bacteria from roots,stems and leaves volatile oil was greater than that of gram-negative bacteria,moreover,the MIC of 10 batches of volatile oil from Angelica stems and leaves was generally lower than that of volatile oil from Angelica rootsPartial least squares(PLS)regression method and bivariate correlation analysis were used to establish the spectrum-effect relationship between volatile oil and antioxidant and antibacterial efficacy,and the material base related to efficacy foundation was screened out for the first time.The results showed that(1)the efficacy foundation of the antioxidant effect of the volatile oil fromAngelicasinensis rootswas the compounds represented by peak No.48 and 26.The volatile oil from the stems and leaves of Angelicasinensis preliminary screened out showed that peak No.33,30,11 and 5 were the compounds with strong correlation which contributed more to the antioxidant activity.(2)The efficacy foundation of Angelicasinensisroots volatile oil acting on Escherichia coliwas the compounds represented by peak No.1 and 25;the efficacy foundation for Streptococcus pneumoniae might be the compounds represented by peak No.14,26,and 40;the compounds represented by peak No.6 and 59 were the efficacy foundation of Staphylococcus aureus;compounds of the roots volatile oil with high contribution acting on Bacillus subtiliswere compounds with peakNo.2,4,5,30,25,50,21 and 36,and compounds with strong correlation were compounds with peak No.60,59,32,53,26 and 41;the compounds with high contribution acting onPseudomonas aeruginosa were compounds with peak No.2,14,16,57,21,58,25,and 16,while the compounds positively correlated to the action of Pseudomonas aeruginosa were compounds with peak No.26,38,37,41,32 and 40;the efficacy foundation of the volatile oil from Angelica roots acting onSalmonella paratyphiB might be the compounds represented by peak No.65 and 41.(3)The efficacy foundation acting on Escherichia coli in the volatile oil from Angelicasinensis stems and leaveswas the compounds represented by peak No.1;peak No.2 and 31 were the efficacy foundation acting onStreptococcus pneumoniae in the volatile oil from Angelicasinensis stems and leaves;for Staphylococcus aureus,the efficacy foundationof the preliminary screening out was the compounds represented by peak No.11,47,35 and 21;the compounds of the volatile oil from the stems and leaves with greater correlation to Bacillus subtiliswere compounds with peak No.1 and 5;the compoundsof the volatile oil from the stems and leaves represented bypeak No.21,58,20,9,57,2,12 and 19 were the efficacy foundation acting onPseudomonas aeruginosa;the efficacy foundation of the volatile oil fromAngelicasinensisstems and leaves acting on Salmonella paratyphiBwas compounds represented by peak No.20,30,33,36,38 and 39.The types and content of chemical composition of volatile oil from Angelicasinensis roots,stems and leaves are quite different,so are their antioxidant and antibacterial activities.The establishment of the spectrum-effect relationship clarifies that the pharmacodynamic action of the volatile oils from roots,stems and leaves of Angelicasinensis are the results of the combined action of various compounds.This experiment provides certain theoretical basis for the development and utilization of Angelicasinensisresources... |
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