Preliminary Study On Antioxidant And Chemical Constituents Of Volatile Oil From Syringa Velutina Kom Leaves

ObjectiveTo optimize the extraction technology of volatile oil from Syringa velutina Kom leaves and analyze the chemical composition of volatile oil from Syringa velutina Kom leaves.Besides,to study the antioxidant activity of volatile oil from Syringa velutina Kom leaves.Provide the reference of volatile oil from Syringa velutina Kom leaves quality control and antioxidant activity.MethodsExtract volatile oil from Syringa velutina Kom leaves through steam distillation and use single factor analysis method and Orthogonal experiment to optimize three extraction factors such as soaking time,adding water,extraction time.Then determine optimize the extraction technology by volatile oil from Syringa velutina Kom leaves oil rate.Analyze the chemical composition and content of volatile oil by using GC-MS chromatographic.Study the antioxidant capacity of volatile oil from Syringa velutina Kom leaves through the scavenging ability of DPPH free radical.ResultsThe best extraction condition of volatile oil from Syringa velutina Kom leaves by steam distillation is: do not soak,multiples for 40 times of water,extract 6 h.Volatile oil from Syringa velutina Kom leaves in a total of 39 compounds were isolated,accounting for 78.51% of the total content: linalool,a-terpineol,damascone,2,3-twohydrogen-1,1,5,6-tetramethyl-1H indene,palmitic acid and dibutyl phthalate etc.Inside,linalool 8.31%,alpha terpineol alcohol 3.85%,turkic ketone 2.88%,palmitic acid 10.32%.In the oxidation experiments,DPPH clearance ability was enhanced by the concentration increased of volatile oil,which was positively correlated.ConclusionsThe extraction of volatile oil from Syringa velutina Kom leaves by steam distillation is feasible.Volatile oil from Syringa velutina Kom leaves confirmed a variety of active ingredients,which has an important significance on pharmacological research.Determine linalool,alpha terpineol,damascone,palmitic acid 4 active ingredients of volatile oil from Syringa velutina Kom leaves content by GC-MS.The method is conveninet,good reproducibility and high accuracy.Volatile oil from Syringa velutina Kom leaves has a certain antioxidant capacity,which provide basic research of volatile oil from Syringa velutina Kom leaves quality control and further study.