Effects Of RNA Interference Targeting HTERT Gene On The Migration And Invasion Of The Human Malignant Melanoma Cell Line A375

Objective: To target the interference of hTERT gene expression in melanoma A375 cells with specific siRNA and observe the effect on the migration and invasion ability of melanoma cells,in order to explore the role of hTERT gene in the pathogenesis of melanoma.Methods: Culture melanoma A375 cells,and set up blank control group,negative control group and interference group respectively.The interference group used specific hTERT siRNAs screened by the research group to transfect melanoma A375 cells,and the negative control group used negative control siRNA to transfect the cells.The blank control group cells were not treated.Real-Time PCR and western experiments were used to detect hTERT m RNA and protein expression levels in each group of cells.Cell scratch and Transwell experiments were used to detect the cell migration and invasion ability of each group before and after interference.Results: Targeted interference with hTERT gene using specific hTERT siRNA can significantly down-regulate the expression of hTERT gene m RNA and protein,and the difference is statistically significant compared with the negative control group and the blank control group(P <0.05).The results of the cell scratch test and the Transwell invasion test showed that when the expression of hTERT gene was silenced in A375 melanoma cells,the migration and invasion ability of the cells also decreased significantly(P <0.05).Conclusion: Using specific hTERT siRNA can effectively silence the expression of hTERT gene in melanoma A375 cells,and the migration and invasion ability of melanoma A375 cells were reduced after hTERT gene was silenced.