| Objective:To evaluate the therapeutic effect of hepatocyte transplantation(HCT)on rat liver cirrhosis with small-for-size syndrome(SFSS).Methods:The liver cirrhosis rat model was established by subcutaneous injection of analytically pure carbon tetrachloride and alcohol drinking.The process lasted 14 weeks,and the liver cirrhosis SD rat model was successfully established.A total of 30 cirrhotic SD rats were randomly divided into Group A(liver cell transplantation 3 days before surgery,n=10),Group B(liver cell transplantation during surgery,n=10),and Group C(blank control group,n=10)and underwent 70%liver resection.The general conditions,survival rate,blood biochemistry,changes in related genes and protein levels and liver histology of rats in each group were observed after surgery.Variance analysis was adopted for comparison between groups of measurement data conforming to normal distribution.Further comparison between two groups,LSD-t test was used for variance equality and Games-Howell test was employed for variance inequality.Log-rank test was used for survival rate comparison.Results:The recovery time of rats in Group A was less than 1 hour after operation,drinking water and a little food were observed 12 hours after operation,and rats'autonomous activities were observed 24 hours after operation.Group B rats had the longest waking time of about 2 hours,drinking water 24 hours after operation and autonomous activities 36 hours after operation.The recovery time of Group C rats was about 3.5 hours at the longest,and drinking water was given in small amount 24 hours after operation,and voluntary activities were observed 48 hours after operation.The survival rate of Group A(90%,9/10)was significantly higher than that of Group B(50%,5/10)and Group C(30%,3/10).The survival rate log-rank test?~2=6.440,p=0.04,with statistical difference.The mean ALT values of Group A,Group B and Group C were 860.1±39.9U/L,904.6±35.6 U/L and 927.4±17.0 U/L 24 hours after operation respectively(F=6.808,p=0.007);AST mean values:896.3±44.7 U/L,923.8±31.6 U/L,950.0±16.3U/L(F=3.666,p=0.047);ALB mean values:25.6±0.5 g/l,24.8±0.6 g/l,23.4±0.4 g/l(F=26.577,p=0.000).Group A was better than the other two groups,and the recovery speed of Group A was faster than that of the other two groups.Up to 240 hours after operation,the mean ALT values of the three groups were 97.8±10.0 U/L,128.2±20.7U/L,150.5±14.8 U/L respectively(F=13.816,p=0.001);AST mean values:108.7±10.8 U/L,142.0±14.1 U/L,161.0±21.2 U/L(F=17.220,p=0.000);ALB mean values:29.1±0.6 g/l,28.0±0.2 g/l,27.0±0.2 g/l(F=15.629,p=0.001),all with statistical significance.The relative expression of IL-6 and TNF-?m RNA in Group A was significantly lower than that in Group B and Group C 72 and 240 hours after operation,but there was no significant difference in IL-1.The expression of IL-6 and TNF-?protein in Group A at 72 and 240 hours after operation was also significantly lower than that in the other two groups.A total of 72 hours after operation,pathological sections of rats in Group C showed disappearance of hepatic cord structure,necrosis of large hepatocytes and infiltration of inflammatory cells.In group B,the arrangement of hepatocytes was disordered,with severe edema,inflammatory cell infiltration and punctate necrosis of hepatocytes.In Group A,only cell balloon-like lesions,punctate cell necrosis and a small amount of inflammatory cell infiltration were observed.\Conclusion:1.Hepatocyte transplantation can effectively promote the recovery of liver function and improve survival rate of postoperative small-for-size syndrome in cirrhotic rats;2.The therapeutic effect is related to the time point of cell transplantation;3.Hepatocyte transplantation technology can provide a new therapeutic method and idea for the treatment of liver failure after clinical surgery. |
PDF Full Text Request