Allogeneic Rat Liver Cell Transplantation For Treatment Of 90% Liver Resection Surgery-induced Acute Liver Failure

Objective: The aim of this study was examination of the beneficial efficacy of intrasplenic syngeneic hepatocyte transplantation(HT) on 90% hepatectomy-induced acute liver failure(ALF) in rats and time-related efficacy of HT in acute liver failure. Methods: Rat hepatocytes were isolated and purified according to a modified two-step purfusion method of seglen~11, Which were transplanted into the rats in which 90% of liver lobes were resected. Results: Viability of isolated hepatocytes was 95% by typan blue exclusion. The Number of harvested hepatocytes per gram of liver tissue was 2.8 >K 108. lntrasplenic tranplantation of ten million isolated fresh hepatocytes 3 days before operation can significantly improve the resistibility of the rats with ALF, the survival rate of rats was significantly higher than that of those whose hepatoeytes were transplanted at the same time as hepatectomy and that of those on which only a hepatectomy was performed. Histological examination of the spleen revealed hepatocytes maintained a normal structure and kept alively. TBiL, SGPT and SGOT plasma analyses of experimental group and control group showed statistics difference. Conclusions: There existed significant effects of intrasplenic hepatocytes transplantation on surgical-induced ALF if performed prior to hepatectomy. HT may be a good alternative treatment for terminal hepatic diseases.