| Objective: This study takes liver fibrosis model rats as the research object,based on the therapeutic effect of Fuzheng Huayu Recipe and Xuefu Zhuyu Recipe on liver fibrosis,and explores the use of multimodal ultrasound technology in evaluating liver fibrosis by promoting blood circulation and removing blood stasis The application value of reversal and the therapeutic effect of Fuzheng Huayu combined with Xuefu Zhuyu application.Methods: Fifty-three male Wistar rats were randomly divided into five groups according to average body weight: normal group(Control),model group(DMN),Fuzheng Huayu group(FZ),Xuefu Zhuyu group(XF),Fuzheng United Blood House Group(FZ+XF).After pre-experiment,intraperitoneal injection of 0.5% dimethylnitrosamine 2?l/g,3 days a week for 4 weeks,to prepare an early liver cirrhosis model.After successful modeling,gavage treatment was performed: the FZ group was administrated with Fuzheng Huayu capsule solution at 473 mg/kg rat body weight,the XF group was administrated with Xuefu Zhuyu capsule solution at 504 mg/kg rat body weight,and the FZ + XF group At the same time,the body weight of 977mg/kg rats was intragastrically administered with Fuzheng Huayu Capsule and Xuefuzhuyu Capsule solution,and the Control group and DMN group were intragastrically fed with the same volume of distilled water.In the 8th week,ultrasound examination was performed.The liver was scanned with conventional two-dimensional ultrasound,and the liver morphology,liver parenchymal echo,portal vein walking,observation of portal vein inner diameter,spleen length,and thickness were measured;portal vein was detected by ultrasound color Doppler technique Flow rate;Observe the color change of liver parenchyma in liver fibrosis rats using ultrasound elastography mode;use ultrasound contrast mode,start ultrasound built-in timer technology during the injection of contrast agent,and observe portal vein and liver parenchyma The whole development process of imaging,storage and development process,using the contrast analysis software that comes with the ultrasound machine to quantitatively analyze the time intensity curve of liver parenchyma and portal vein,analyze the quantitative parameters Time to peak(TTP),peak intensity(PI),Area under curve(AUC),Ascending Slope(AS),Descending Slope(DS),Halve lasting peak(HIP),Mean transit time(MTT),initial development time(AT),acceleration Time(ATT).After the ultrasound examination,the rats were sacrificed,the liver and spleen were weighed,and the liver-body ratio and spleen-body ratio were calculated;the rat liver tissue specimens were fixed for HE and Masson staining to observe liver pathological changes;Rat serum was taken to measure the content of alanine aminotransferase(ALT)and aspartate aminotransferase(AST);ELISA method was used to detect hyaluronic acid(HA),laminin(LN),type III procollagen(PC?),type IV collagen(C?)Content;immunohistochemistry(IHC)detection of ?-smooth muscle actin(?-SMA),transforming growth factor-?1(transforming growth factor-?1,TGF-?1)in rat liver Medium protein expression.Results:(1)During the modeling period,the weight of the model-building rats was significantly lower than that of the normal group,the weight of the model-building rats showed a downward trend,and the body weight of the Control group of rats increased.(2)The liver body ratio of DMN group was significantly lower than that of Control group(P < 0.05),and the spleen body ratio was significantly higher(P<0.01).FZ group was able to significantly increase the original reduce liver body ratio at while XF group was able to significantly reduce the original increased spleen body ratio.(3)HE and Masson staining showed that the control group had a complete hepatic lobule structure,normal cell morphology,clear liver sinuses,and no obvious fibrous tissue hyperplasia.In the DMN group,the structure of the liver lobule was disorderly changed,the cells were swollen and necrotic,and the pseudolobule was formed with a lot of fibrous hyperplasia.This indicates that the rat liver fibrosis model is successfully established.Compared with the DMN group,the liver pathological changes of the three groups of drug treatment groups were significantly improved.Masson staining showed that Collagen Volume Fractio(CVF)in the DMN group increased significantly(P <0.01) compared with the Control group,and the FZ group could significantly reduce the original collagen volume fraction.(4)The contents of HA,LN,PC?,and CIV in the DMN group increased significantly compared with the Control group(P <0.01).The FZ group could significantly reduce the original increased HA and CIV content,and the XF group could significantly reduce the originally increased LN content,FZ +.The XF group can significantly reduce the original increased PC? content.(5)The content of ALT and AST in the DMN group was significantly increased(P <0.01 or P <0.05),the FZ + XF group could significantly reduce the original increased ALT content,and the FZ group could significantly reduce the original increased AST content.(6)The portal vein inner diameter of the DMN group was significantly increased and the portal vein flow rate was significantly slower than that of the Control group(P <0.01).The FZ + XF group was able to significantly reduce the originally increased portal vein diameter and significantly accelerate the originally slowed portal vein flow rate.The spleen thickness in the DMN group was significantly thinner and the spleen length was significantly increased compared with the Control group.From a trend perspective,the spleen thickness in the XF group and the spleen thickness and length in the FZ + XF group were closer to the Control group values,but there was no statistical significance.Quantitative parameters of time intensity curve of liver parenchyma: The peak intensity(PI),area under curve(AUC),ascending slope(AS),descending slope(DS)and time to peak(TTP)of DMN group were significantly lower than those of control group(P < 0.01).XF group could significantly increase the original decreased peak intensity(PI),area under curve(AUC),descending slope(DS)and shorten the original extended time to peak(TTP).FZ group could significantly increase the peak time(TTP)The slope of rise(AS)of the original decrease was obviously increased.Quantitative parameters of time intensity curve of portal vein: the peak intensity(PI)and area under curve(AUC)of DMN group were significantly lower than those of control group(P < 0.01),and FZ + XF group could significantly increase the original decreased peak intensity(PI)and area under curve(AUC).Compared with the control group,the rising slope(AS)and falling slope(DS)of DMN group decreased,and the time to peak(TTP)of XF group was longer.From the trend,the rising slope(AS)of FZ group and the falling slope(DS)of FZ + XF group were closer to the control group,and there was no significant difference.The sensitivity,specificity and accuracy of elastography were 87.1%,62.5% and 82.05%,respectively.The area under ROC curve was 0.855(P = 0.002).(7)The expression levels of ?-SMA and TGF-?1 protein in the DMN group were significantly increased compared with the Control group(P <0.01).The XF group could significantly reduce the original increased ?-SMA protein expression level,and the FZ group could significantly reduce the original increased TGF-?1 protein expression level.Conclusion:(1)Through the observation of liver morphology,hemodynamics,and hepatic vascular microcirculation,it can be concluded that multimodal ultrasound technology has a good application value for reversing liver fibrosis in rats by promoting blood circulation and removing blood stasis.(2)The therapy of promoting blood circulation and removing blood stasis is helpful for reversing liver fibrosis in rats,and the curative effect of Xuefu Zhuyu and Fuzheng Huayu combined with Xuefu Zhuyu is better than Fuzheng Huayu. |
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