Construction Of Intracranial Drug Stent And Its Effect On Promoting Endothelialization

Background and purpose At present,the stents used in neurosurgical interventional surgery for the treatment of intracranial aneurysms are bare stents,which cannot promote the endothelialization of the neck of aneurysms containing tumors,which leads to the recurrence rate of aneurysms after stent treatment.Still higher than 10%.Therefore,how to make the stent more capable of promoting the endothelial repair ability of the neck of the aneurysm is the key to improve the occlusion rate of the aneurysm and reduce the recurrence rate of intravascular interventional treatment of the aneurysm.We conceived that loading a bare scaffold with vascular endothelial growth factor(VEGF)that helps promote endothelialization and anti-CD34 antibody that can effectively adhere to endothelial progenitor cells,so that it can better promote the growth of tumor-bearing arterial vascular endothelial cells.Methods First,we simulated the construction of drug-eluting stents using a nickel-titanium alloy sheet of the same material as the bare stent.Using the self-polymerization reaction of dopamine and the strong adhesion properties of mussel-like protein,a layer of polydopamine was fixed on the flat plate of nickel-titanium alloy,and then heparin and vascular endothelium were fixed on the surface of the dopamine layer by covalent bonding and in situ reduction.Growth factors and anti-CD34 antibodies.After verifying successful loading of vascular endothelial growth factor(VEGF)and anti-CD34 antibodies by cy3 labeled streptavidin,human VEGF Elisa kit experiment and human Ig G Elisa kit experiment,water contact angle experiment and protein adhesion experiment,Clotting time,platelet activation experiments and other experiments proved that the improved nickel-titanium alloy flat film has better blood compatibility.Then,we verified that the human umbilical vein endothelial cell(HUVEC)proliferation experiment,migration experiment and human endothelial progenitor cell(EPC)capture experiment showed that the improved drug nickel-titanium alloy sheet had better effect on endothelial cell proliferation and capture than the bare flat sheet.Theability of endothelial progenitor cells.We used the same method to construct a new intracranial drug scaffold,and observed the scaffold loaded with VEGF and anti-CD34 antibodies simultaneously with a fluorescent antibody under a laser confocal microscope,and performed HUVEC cell proliferation experiments and human endothelial progenitor cells(EPC)Cell capture experiments.Results The improved new intracranial drug-eluting stent has better blood compatibility and ability to promote vascular endothelialization than naked stent.Conclusion The new intracranial drug-eluting stent is theoretically superior to today's bare stent in solving the problem of high recurrence rate of aneurysm due to insufficient endothelialization of aneurysm-bearing aneurysm neck.