Preparation Of Chloroplast Light-harvesting And Oxygen-releasing Coating Materials And Research And Development Of Gas Monitoring Devices

Photosynthesis refers to the process by which green plants and algae absorb light energy,synthesize carbon dioxide and water into rich organic matter,and release oxygen.As a site for photosynthesis,chloroplasts use their chlorophyll to transform light energy into chemical energy.At present,the construction of new materials for simulating plant photosynthesis to capture solar energy has attracted wide attention of the scientific community.In recent years,with the development of semiconductor photocatalysis technology,its excellent photocatalytic activity and stability in photocatalytic reactions have attracted a lot of interest from researchers in the field of catalysis,and have obtained a lot of research results.It includes metal-semiconductor composite photocatalyst and organic-semiconductor composite nano-aggregate photocatalyst and so on.However,there are some problems in the construction of the composites,such as complex system design and complicated preparation.Therefore,based on a large number of literature reports,the research group proposed a material construction method using live chloroplast as the main matrix and compounding inorganic photocatalyst.To achieve the light-harvesting and oxygen-releasing properties of the composite,a novel composite material was designed and prepared.In order to study the light-harvesting and oxygen-releasing properties of materials,we developeda gas concentration monitoring experimental device to track and record the oxygen released from chloroplast composites.The main content of this work is divided into the following two parts:(1)Using chloroplast as the main matrix and compounding MnO2 and MnCl2 catalysts,the chloroplast coating materials were prepared,and the light-harvesting and oxygen-releasing properties of the composites were studied.The morphology of the chloroplasts was observed by scanning electron microscopy(SEM).The results showed that the chloroplasts were structurally intact,well dispersed,uniform in particle size and non-adhesive.The coating materials were prepared by energy dispersive spectroscopy(EDS).Analysis of the elemental distribution shows that the MnO2 particles and Mn are uniformly dispersed on the surface of the chloroplast,and have a good composite effect.Finally,the composite coating material was tested by UV-vis spectrophotometer(UV-vis)and self-developed gas-concentration monitoring device.The experimental results show that the composite coating material has certain light-harvesting and oxygen-releasing properties.(2)In view of the problem that the existing oxygen electrodes in the domestic and international markets cannot meet the monitoring released oxygen from large-area coating materials,we have developed a multifunctional gas concentration monitoring device.The device is equipped with xenon lamp as a visible light source,installed with oxygen and carbon dioxide gas detection module.The device can monitor the light-harvesting and oxygen-releasing properties of the composite coating material prepared in the above work.In addition,it can also be applied to the monitoring of other substances or gases to be tested.It can monitor a variety of gases at the same time.The vacuum mode can be startuped according to the experimental requirements.In this work,the device was used tomonitor the oxygen-releasing properties of chloroplast/Ti02,chloroplast/MnO2,chloroplast/MnCl2.The results showed that the trend of the oxygen release of the chloroplast suspension was consistent with the experimental results obtained from the UV-vis spectrophotometer(UV-vis)analysis and from the Clark oxygen electrode detection.The accuracy and reliability of experimental data are proved.