Research On In-situ Ionization Mass Spectrometry Analysis Method Of Mainstream Cigarette Smoke Components

Cigarette smoke is an extremely complex chemical system.The chemical composition of the mainstream smoke(MMS)contains thousands of compounds existing in a broad concentration range.Mainstream smoke(MSS)contains various toxic and carcinogenic substances,which is directly inhaled into the human body,and poses risk to human health.Studies have indicated that smoking is closely related to the occurrence of lung cancer,and is also associated with many other diseases,such as cardiovascular disease,stroke,chronic bronchitis etc.Therefore,it is of significance to determine the particular phase of MMS.However,due to the complexity of the cigarette smoke,it is difficult to measure different chemical constitutes of cigarette smoke simultaneously,which poses great challenge for analytical techniques.Therefore,the development of a simple and efficient analysis technology is essential for the profound exploration of MMS.The concrete research content is as follows:In the first part,ambient ionization mass spectrometric method of direct analysis in real time mass spectrometry(DART-MS)was used to rapidly and simultaneously measure the multiple particulate components in MSS.With this technique,a large number of compounds containing various types of functional groups were fragmented and detected in seconds.The optimal experimental conditions of 300 ℃ and 0.2 mm/s were selected by comparing the different experimental conditions.The particulate matter extracts of MSS from different cigarettes using the same extraction solvents were investigated by DART-MS.In order to further explore the differences among the various extracts,principle component analysis(PCA)was performed.It was thus demonstrated that DART-MS could measure the multiple chemical constitutes in the particulate matter of MSS in a fast,simple and efficient manner,which serves as a powerful tool for the acquisition of the fingerprint library for complex samples and characterization of the components in the sample as well.In the second part,qualitative and quantitative analysis of the MMS particulate phase based on DART-MS and Ultra-performance liquid chromatography mass spectrometry(UPLC-MS)was carried out.The chemical formulas’assignment and compound identification were proceeded based on accurate m/z values with mass errors less than 10ppm.Accurate quantitative of nicotine contents was performed with DART-MS and the results were validated by UPLC-MS,the contents of nicotine obtained by using the two methods were the same.It turns out that DART-MS can not only evaluate complex sample components(such as soot particles)easily and efficiently,but also achieve the accurate quantification.In the third part,DART-MS combined with Solid-phase micro extraction(SPME)were used to further explore particulate phase of MMS,and the results indicated that this method could reduce the matrix effect in complex samples.The conditions of 20 minutes,2 times dilution,350 ℃,and 0.2 mm/s were obtained by optimizing the experimental conditions.C18 coating probe had a better extraction efficiency by comparing and analyzing six different MMS particulate with different adsorption coatings.At last,the adsorption probe was desorbed several times in succession,and it was found that the first desorption can achieve effective mass transfer.This study shows that DART-MS combined with SPME technology not only has the advantage of reducing matrix effects in complex samples,but also may increase the signal intensity of some detection ions.which provides a new idea for the analysis and research of complex sample matrix.In summary,this study uses a new detection method(DART-MS)to conduct the profound research on MMS particulate matter.The research contents include the establishment of DART-MS;the determination of the absolute molecular weight of the main components of MMS and the quantitative analysis of nicotine components;and the research of DART-MS coupled with SPME technology.