Study On The Degradation Law And Biological Safety Of A New Type Of Bile Duct Stent Mg-Nd-Zn-Zr Magnesium Alloy In An In Vitro Bile Perfusion Model

Objective The degradation of magnesium alloy was observed by in vitro simulation of biliary system microenvironment,and the effect of material extract on the value-added activity of L929 mouse fibroblasts was measured by CCK-8 method.Through these two methods to Evaluate its biosafety,and make a preliminary assessment of it.Methods Production wafer and bracket form of JDBM magnesium alloy and magnesium fluoride plus polylactic acid coated,and placed in the bile perfusion system in vitro,regular observation of corrosion,monitoring the quality of change.The effect of stent extract on the proliferation of L929 mouse fibroblasts was then analyzed by CCK-8 method.Result The mass loss rate of the bare magnesium alloy sheet and the stent group was higher than that of the corresponding coating group(p<0.05).There was no significant difference(P>0.05)between the bare magnesium alloy sheet and the stent extraction group,the coated slice extract group and the negative control group.Conclusion The weight loss rate of the coated magnesium alloy sheet and the support group in the JDBM magnesium alloy was significantly lower than that of the bare magnesium alloy sheet and the corresponding support group.The coating had protective effect on the magnesium alloy.JDBM magnesium alloy material and magnesium fluoride plus polylactic acid coating material has good biosafety.