Observation Of The Clinical Effect Of Antibacterial Photodynamic Therapy (APDT) On AIDS Combined With Oral Candidiasis Albicans

Objective Through 54 cases of human immunodeficiency virus(HIV)infection and AIDS(HIV/AIDS)to merge the etiology of oral candida disease identification,clinical phenotype and CD4~+ T lymphocyte count were analyzed.Methods Select 54 cases diagnosed with HIV/AIDS oral candidiasis in hospitalized patients,according to the epidemiology of ?The oral mucosa ? as the diagnostic criteria for their oral candidiasis clinical phenotype,instruct patients with a tendency for 0.1 mmol /L phosphate buffer solution(PBS)rinse your mouth after 3 times,with pharyngeal swab to wipe the lesion mucosa for smear gram staining,microscopy to observe the morphology of hyphae and spores.And the pharyngeal swab samples in CHROMagar candida color medium,placing it in 37 ? temperature cultivation in 24-48 h,observation of candida colony color to identify the etiology.Using EDTA anticoagulant collection tube4-5 ml peripheral venous blood,cells in the detection of peripheral blood CD4~+ T lymphocyte count.Results The most common clinical phenotype of 54 patients with HIV /AIDS combined with pseudomembranous candidiasis 68.52%(37/54).Secondly for the erythema candidiasis is 14.82%(8/54);9.26%(5/54)is angular cheilitiswith pseudomembranous candidiasis;angular cheilitis with erythema multiforme candidiasis is 3.70%(2/54);3.70%(2/54)is proliferative type oral candidiasis.Through CHROMagar medium separation produce 60 strains of candida,candida albicans detection rate was 75.0%,followed by tropical candida 11.67%.CD4~+ T lymphocyte count range of 5 ~ 464 cells/mm~3,With different clinical phenotypes,CD4~+ T lymphocytes count with candida etiology identification results were statistically analyzed,the results hint CD4~+ T lymphocytes count < 50 / mm~3 oral candida albicans sample central Africa of the patients was higher than that of CD4~+ T lymphocyte number ? 50/mm~3,comparing differences between the two groups was statistically significant(P < 0.05).Conclusion Oral candida infection in patients with HIV/AIDS clinical phenotype is given priority to pseudomembranous candidiasis,followed by erythema candidiasis.Candida isolated in mainly candida albicans,followed by tropical candida;The candida albicans for CD4~+ T lymphocyte count < 50/ mm~3 common pathogenic bacteria in patients with HIV/AIDS in Guangxi.Objective TO explore the clinical curative effect of the Dimethylmethylene blue(DMMB)mediated APDT in AIDS with oral candida albicans infection patients.Methods APDT group: Selected 34 AIDS with oral candida albicans infection hospitalized patients for DMMB mediated APDT treatment.Before DMMB mediated APDT treatment,with a tendency for 0.1 mmol /L PBS buffer rinse your mouth after 3 times,in the mucous membrane of the affected area concentration tendency for 1.0mmol/LDMMB hatching after 5 min.The light energy was 10 J/ cm2.Treatment before and after each treatment oral cavity containing wash liquid centrifugal,take the capacity to 1 m L of the precipitation candida albicans suspension liquid,using the gradient dilution method using cell counting board direct microscopy candida albicans bacteria count,calculate per ml contains number of candida albicans bacteria.At the same time take10?L candida albicans suspension inoculation in CHROMagar candida colormedium,compare APDT colony count changes before and after the treatment.According to the different,oral anatomical site of different parts of the treatment effect were analyzed.Fluconazole group: 8 cases in hospitalized patients diagnosed with AIDS oral candidosis white,oral fluconazole 100mg/day,treatment for 2 weeks.Results(1)APDT can effectively kill the AIDS patients of oral candida albicans,DMMB mediated APDT 1 time after treatment compared with before treatment,the survival rates of candida albicans is 53.96%;After second treatment survival rate was 39.12%;3 times after treatment of candida albicans survival rate is 22.75%;Every time before and after treatment comparison between candida albicans bacteria number difference was statistically significant(P <0.05).(2)According to different anatomical site statistics,a total of 53 in mucosal disease area,continuous line DMMB mediated APDT 3 times after treatment,pathological changes of tongue,bilateral buccal film cure rate comparison,the difference was statistically significant(P<0.05).(3)The age of APDT and fluconazole group(t = 2.10,p = 0.06),gender(?_C~2 = 0.0046,p >0.05),who clinical stage(?_C~2 = 0.13,p > 0.05),CD4~+ T lymphocyte count(t =0.07,p = 0.94),there was no significant difference(p > 0.05).In APDT group41.18% patients recovered,effective rate was 97.06%;The fluconazole group cure rate was 87.5%,effective rate was 100%.APDT and fluconazole drugs group cure rate comparison,the difference was statistically significant(P < 0.05).Conclusion(1)DMMB mediated APDT merger in patients with AIDS oral candida albicans has good killing effect.(2)Line APDT treatment after 3 times in a row,bilateral buccal membrane lesions of cure rate higher than the back of the tongue.(3)APDT group compared with the effect of fluconazole treatment group,fluconazole group cure rate is higher than the APDT.