Based On The In Vivo Distribution Method Of Effect Components, The Relationship Between Evodia And Scutellaria Baicalensis Was Studied

[Objective]:Through the evaluation of the association between the channel tropism and the targeting of drug pair Evodia rutaecarpa and scutellaria,evaluation of the nature of "led by the herald" for Evodia rutaecarpa,serve as a reference for clinical combination.[Methods]:1.To find and extract the records of the channel tropism and meridian-guiding of Evodia rutaecarpa in the ancient Chinese materia medica literature,and analysis and collation.2.The quantitative analysis of Evodiamine,Rutaecarpine and Baicalin inthe different extract powder were carried out by HPLC.3.Based on the in vivo distribution method of effect components to evaluate the association between the channel tropism and the targeting of Evodia rutaecarpa,and the establishment of the effective components of the UPLC-MS/MS analysis method,and the calculation analysis of tissue distribution and the drug-time curve.4.The plasma concentration in the different compatibility proportion of cases and the plasma quantitative analysis method of Baicalin at the same time was established,the plasma concentration,drug-time curve and the main pharmacokinetic parameters were evaluated Pharmacokinetics of baicalin in the Evodia rutaecarpa.5.The tissue concentration of Baicalin in the different compatibility proportion of cases was dynamically changed by UPLC-PDA detection technique,and the tissue quantitative analysis method of Baicalin was established.The tissue content and drug time curve were analyzed and the targeting analysis was carried out,evaluating in vivo targeting effect of baicalin by with Evodia rutaecarpa.[Results]:1.Through the collection of herbal literature,induction analysis,found that Evodia rutaecarpa's channel tropism classified by from high to low are:liver>spleen>stomach>intestine>kidney>lung.The meridian-guiding is the Liver Meridian.The channel tropism and meridian-guiding of Evodia rutaecarpa with the activity of the liver are closely related.2.The establishment of Evodiamine and Rutaecarpine,Baicalin method to verify,in line with the requirements of chemical composition in vitro testing,and the content of different formulations to determine the average content of Evodiamine 3.12 mg.g-1,the average content of Rutaecarpine was 3.09 mg.g-1.The different group with(0:1),(1:2),(1:1)and(2:1)content of Baicalin in points were 294.04,199.63,159.17 and 110.95 mg.g-1.3.The establishment of liver tissue analysis methods of Evodia rutaecarpa's effect composition,to meet the requirements.At the same time point,the content of liver tissue and AUC of Rutaecarpine were higher than that of Evodiamine.4.To establish a method for the determination of Baicalin in plasma,meet the testing requirements.Through the different compatibility proportion of Baicalin with the plasma concentrations,drug-time curve,found that after compatibility of blood concentration in the starting time is significantly lower than the compatibility,inhibition of Baicalin in the upper small intestine absorption;the trend that the remaining time point of blood drug concentration is basically the same.The pharmacokinetic parameters analysis shows that after the compatibility of each group was significantly change the Baicalin's peak time and peak concentrations,and the trend was prolonged and decreased.5.The method of UPLC analysis of Baicalin in the tissues of liver,lung and kidney was established.According to the distribution of Baicalin in the liver,lung,kidney,draw drug-time curve of the same organs of different proportions of medicine also that,at the beginning of time,and Baicalin's concentiration of after each group less than before,And evaluating and calculating the different organs of AUC target-based assessment,compatibility of the liver has no obvious effect,the lung and kidney have certain trgeting and selective function,Evodia rutaecarpa on Baicalin has no effect with "drug Into the liver" role.[Conclusion]:Based on the experiment results,the absorption in the gastrointestinal tract and distribution of Baicalin in the liver,lung and kidney,and then influenced the targeting of the model drugs.From the relative intake rate,the total target coefficient and comprehensive evaluation of drug distribution efficiency,Baicalin on liver has no obvious targeting selectivity.The results showed that the selectivity of Evodia rutaecarpa may be specific to the model drug,and its guiding effect on specific components was not obvious broad-spectrum guiding role.