Based On TGF-?-induced Epithelial-mesenchymal Transition (EMT) Of Human Lung Cancer Cells, The Effect Of Single-faced Needle Alkaloids On Lung Cancer Metastasis Was Studied

Objective:Lung cancer is one of the malignant tumors that threaten human health.According to the American Cancer Society,the global rate of lung cancer metastasis is the highest in cancer,and 80%of them are non-small cell lung cancer?NSCLC?^[1].In recent years,studies have found that epithelial-to-mesenchymal?EMT?of lung cancer cells is closely related to the invasion and metastasis of lung cancer^[2].It involves a variety of cell biological behaviors such as decreased adhesion between tumor cells,degradation of tumor extracellular matrix,and increased tumor cell invasion^[2,3].This study investigated the effects of alkaloids in single-sided needles on NSCLC,and selected its main active compound?nitrazine chloride?to investigate its effect on invasion and metastasis of EMT A549 cells and its mechanism.Methods:The effect of alkaloids in single-sided needles on A549 cells was mainly CCK-8 method.In the process of invasion and metastasis of A549,EMT A549 and chlorinated amphoteric drugs,Transwell and cells were mainly used.The method of scratching was carried out;the nude mice were used to study the tumor sections of nude mice,mainly by HE staining and immunohistochemistry;the mechanism of chlorinated amphetamine?NC?drugs on EMT A549 cells Among them,high-content analysis,Western blot and RT-PCR methods were mainly used for analysis.Results:In this study,we investigated the effects of alkaloids in unilateral needles on the proliferation of non-small cell lung cancer cells,and screened out the active compound for the treatment of non-small cell lung cancer in unilateral needle plants,chlorinated nitidine?NC?;To investigate the effect of different concentrations of TGF-?on EMT A549 cells at different time points,and to explore the optimal induction condition of T,and to intervene for 48h at 50ng/mL concentration.The inhibition of NC on EMT A549 model was demonstrated by in vivo experiments.It has been shown to inhibit its invasion and metastasis and neovascularization,which has also been verified in in vitro cell experiments;by studying the protein E-cadherin,which plays a key role in it,it is found that NC may restore E-cadherin gene expression or up-regulate E-cadherin protein expression plays a role in inhibiting invasion and metastasis of EMT A549 model.