Alkaloids-sided Needle

The plant which comes from Zanthoxylum dissitum Hemsl belongs to Xanthoxylum.This plant being extensively distributed in southeast of our country and some provinces such as Hubei, Hunan,Guangdong, Guangxi, Shanxi, etc.It grows in mountainous region and forest.Its roots, stem barks,and leaves are used in preparing medicines. In traditional Chinese medicine,it tastes pungent, astringent and slightly poisonous.The therapy by using it to cure injuries from falls, wrench, bone fracture and pain arising from stagnation of vital energy.In recent years,the research on cultivation,exploitation and utilization about plants of Xanthoxylum is concentrating on Zanthoxylum nitidum (Roxb.)DC. and Zanthoxylum nitidum var. fastuosum but little investigation on the Zanthoxylum dissitum Hemsl.It is commomly used by local people for a long time and is main constituent of lots of Chinese formulated products.It possess extensive value in medicine and has the therapeutical effect on a lot of disease. So the purpose of this topic is to provid the material foundation of research on anti-inflammatory activity about the Zanthoxylum dissitum Hemsl by studying of alkaloid in it. Accordingly,expand research thought and offer important basis of clinical application concerning further research on the Zanthoxylum dissitum Hemsl. After the phytochemistry research of the Zanthoxylum dissitum Hemsl these compounds have been isolated:Dictamnine,β-sitosterol,β-Daucosterol,3,4-dimethoxyethane-3,4epoxy-furanocoumarin,lupeol, neohesperidin, isoimpinellin, ursolic acid.The topic uses 0.5% HCl to extract the Zanthoxylum dissitum Hemsl, then alkalify the acid liquor by ammonia water and adjust the PH span from 10 to 11. Precipitation appear through standing.Get the precipitation by vacuum filtration or centrifugalization, dry it.Then extract the precipitation by CHCl3, EtOAc, MeOH in order. From the three part of extract the precipitation by normal pressure aluminium oxide column chromatography and dissolvent recrystallisation many times,9 constituents were isolated. On the basis of NMR,MS,IR spectroscopic analysis methods and chemical methods,4 compounds were identified as Nitidine, Sanguinarine, Zanthobungeanine, Dictamnine,Otherwise, this topic makes the cytoactive screening test of these alkaloid in order to provid theoretical basis for research of the effect of alkaloid upon extraorgan cells.