Study On The Preparation Process Of 4-methylsulfinyl-3-butenyl Glucosinolate And Its Hydrogenated Product In Scutellaria Serrata

The seeds of Raphanus sativus L.are dry and mature seeds of Raphanus sativus L.which is a cruciferous plant.It can benefit to digest,swell,lower gas and remove phlegm.It is used for diet stagnation,abdominal pain,constipation,stagnation,phlegm and blood stasis,hemorrhoids cough and other symptoms.This paper mainly studies the preparation process of Glucoraphenin in the seeds of Raphanus.sativu.s L.Based on the above,the application of Glucoraphenin is studied,including hydroconversion.It is the necessity and feasibility of the famous anti-cancer anti-cancer prodrug glucosinolate and Glucoraphenin as the chemical reference substance of the seeds of Raphanus sativus L..Part ? Literature ReviewIt laid the foundation for subsequent research work by reviewing various aspects,including area,glucoraphanin,glucoraphenin,and pharmacological effects of the seeds of Raphanus sativus L..Part ? Screening of the Sources of the Best Medicinal Materials of the seeds of Raphanus sativus L.An HPLC method for simultaneously determining the content of glucoraphenin and glucoraphanin in The seeds of Raphanus sativus L.was established.In order to ensure the feasibility,the chrormatographic and preparation conditions were optimized,which includes the choice of column and detection wavelength.Welch Ultimate LP-C18 column and the wavelength 224 nm were determined.By investigating the enzymatic treatment and extraction method,the solvent preheating enzymatic method and boiling water reflux extraction were determined as the optimal method.On this basis,the optimal preparation method was determined by single factor method.Through the methodological investigation,the contents of glucoraphanin and glucoraphenin in the seeds of Raphanus sativus L.from five producing areas were determined.Finally,the S5 batch from Gansu was selected as the source of the seeds of Raphanus.sativus L.in this study.Part ? Preparation of Glucoraphenin from the seeds of Raphanus sativus L.The preparation process of Glucoraphenin in the seeds of Raphanus sativus L.was investigated comprehensively,including1.Study on extraction technology of Glucoraphenin from the seeds of Raphanus sativus L.Three factors affecting the extraction of Glucoraphenin were carried out by single factor and Box-Behnken response surface design method,which include extraction amount,extraction times and extraction time.In the investigation,the optimal extraction process was finally determined.That was V times of boiling water extracting K for T min each time.2.Study on alcohol precipitation process of the seeds of Raphanus sativus L.water extractThe concentration and volume are the main objects of the alcohol precipitation process.The mass percentage of glucoraphenin in the water extract of the seeds of Raphanus sativus L.after alcohol precipitation was 22.63%,which was 66.71%higher than that of the untreated(13.82%),And the amount of post-emergence extract was reduced by 47.16%.The results show that the optimal alcohol precipitation process has a good initial enrichment effect.3.Optimization of glucoraphenin enrichment process in the seeds of Raphanus sativus L.This section further optimizes the enrichment process for the glucoraphenin.We determined D301 anion resin and acid alumina by examining the effects of five enriched fillers on the recovery of glucoraphenin and the effect of further separation and enrichment.By investigating the loading amount,eluent and elution volume of D301 anion resin and acidic alumina,the optimal enrichment process of the two fillers was obtained respectively.It was found that D301 anion resin is the most suitable for reducing sulforaphane.The process conditions were as follows:A mL drug solution/g,water elution of B BV,and 0.1 mol/L of K2SO4 solution to elute C BV.4.Study on purification process of glucorapheninThe two purification process parameters of C18 column loading and diameter ratio were investigated with the aid of C18 packing,we determined that the optimal purification process was 1:10 loading and the C18 column height ratio was 1:8.After that the purity can reach 98.5%or more.Finally,we have obtained more than 10g glucoraphenin products with a purity of?98.5%.Part IV Study on the Semi-synthetic preparation of glucoraphenin to glucoraphaninWe determined the optimized semi-synthetic preparation process for the conversion of glucoraphenin to glucoraphanin by investigating the type,amount and reaction time of the catalyst.The conditions are as follows:PtO2 was used as catalyst,the catalyst dosage was D%,reaction time is E h.Part V Discussion on the necessity and feasibility of glucoraphenin as the chemical reference substance of the seeds of Raphanus sativus L.The necessity and feasibility that glucoraphenin was as a chemical reference substance for the seeds of Raphanus sativus L.were discussed,which include six aspects.For example,there are efficacy and specificity corresponding relationship between the glucoraphenin and the seeds of Raphanus sativus L.;The sinapine thiocyanate has limitations for the seeds of Raphanus sativus L.as a reference chemical.The feasibility includes the preparation and purity determination of glucoraphenin,good stability to light,temperature and oxidant,perfect content determination and identification methods from the seeds of Raphanus sativus L.