A Study On The Difference Of Serum Metabonomics Between Patients With Angina Pectoris Of Coronary Heart Disease With Phlegm And Blood Stasis Syndrome And Non-phlegm And Blood Stasis Syndrome

Purpose:Using metabonomics through the comparative analysis of intermingled phl egm-blood stasis syndrome of coronary heart diseases with angina pectoris patients and non-intermingled phlegm-blood stasis syndrome of coronary heart diseases with angina pectoris patients,the difference of the mining of intermingled phlegm-blood stasis sy ndrome of coronary heart disease with angina pectoris phlegm specific small molecular markers.Material and method:According to the diagnostic criteria,inclusion criteria and exclusion criteria,we recruited and screened 90 cases of CHD patients with intermingled phlegm and blood stasis and non-intermingled phlegm stasis syndrome from the first affiliated hospital of Jinzhou medical university and traditional Chinese medicine hospital of Yingkou from 2015-2016.Each in the two groups of 30 cases of serum samples were randomly selected for metabonomics liquid chromatography-mass spectrometry(LC-MS)detection,by using principal component analysis and discriminant analysis research of coronary artery disease angina patients with intermingled phlegm-blood stasis by each group and with the intermingled phlegm-blood stasis syndrome in patients with serum metabolites of spectral differences.Using automatic biochemical analyzer to detect serum total cholesterol(TC),triglyceride(TG),high-density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C)levels,Elisa method to detect each group patients serum 5 lipoxygenase(5-LO),leukotrienes B4(LTB4),leukotrienes E4(LTE4)content.Results: 1.According to the general data,there is no obvious difference between the patients with intermingled phlegm-blood stasis syndrome and non-intermingled phlegm-blood stasis syndrome,which is comparable.2.The results shows that the TG,TC and LDL-c levels in the TG,TC and LDL-c levels of the patients with intermingled phlegm-blood stasis syndrome were higher than those in the non-intermingled phlegm-blood stasis syndrome group(P < 0.01).3.The testing results of metabonomics,under the premise that the quality of qualified,coronary heart disease with angina pectoris intermingled phlegm-blood stasis syndrome group compared with the non-intermingled phlegm-blood stasis syndrome group,there are differential metabolites:6-Dehydrotestosterone glucuronide,Glycochenodeoxycholic acid,Leukotrienes E4,Corchorifatty acid A,Arachidonic,PC(22:2(13Z,16Z)/15:0),Perillic acid,Benzoic acid,Leucyl-Leucine and 12-Keto-tetrahydro-leukotriene B4.4.ELISA method to detect the serum content of 5-LO,LTB4,LTE4.The results shows that 5-LO and LTB4 have the similar expression level between the two groups,but the LTE4 content has difference between the two groups(P < 0.01).Conclusion: 1.We found that the differential metabolites between intermingled phlegm-blood stasis syndrome of coronary heart diseases with angina pectoris and non-intermingled phlegm-blood stasis syndrome of coronary heart diseases with angina pectoris,including 6-Dehydrotestosterone glucuronide,Glycochenodeoxycholic acid,Leukotrienes E4,Corchorifatty acid A,Arachidonic,PC(22:2(13Z,16Z)/15:0),Perillic acid,Benzoic acid,Leucyl-Leucine and 12-Keto-tetrahydro-leukotriene B4.2.To elucidate the important differential small molecule metabolites about LTE4 between intermingled phlegm-blood stasis syndrome of coronary heart diseases with angina pectoris and non-intermingled phlegm-blood stasis syndrome of coronary heart diseases with angina pectoris...