| Objective: To investigate the effect of Tangshen’an decoction on Nrf2/ARE pathway in renal tissue of experimental type 2 diabetic rats and to study its protective effects on the kidney.Materials and Methods: Fifty healthy male SD rats were randomly divided into normal group and model building group.Rats in model group were fed with high fat and high glucose.Type 2 diabetes model was established by intraperitoneal injection of streptozotocin(STZ)at a dosage of 35mg·kg-1.Rat models were randomly divided into model group,Tangshen’an low,medium and high dose group and western medicine group.Each treatment group was given the appropriate treatment.After 8 weeks,the general situation in rats was observed.24 h urine microalbumin was detected by collecting urine sample.Fasting blood glucose plasma lipid and renal function were measured by abdominal aorta blood sampling.The kidneys were collected and the expression of Nrf2 and HO-1 protein in kidney were detected by immunohistochemistry.The protein and m RNA expression of NF-κBp65 in kidney were detected by Western blot and RT-PCR.Result:1.General situation of rats in each group: The rats in the model group showed diminished activity,bleak hair color,more drink,more food,sluggishness,unresponsiveness and even bowstring curling body.After 8 weeks,the body weight of the model group and each treatment groups was lower than that of the normal group(P<0.05).2.Changes of Fasting Blood Glucose in rats in each group:After 8 weeks,fasting blood-glucose of the model group and each treatment group was significantly higher than the normal group(P<0.01).3.Changes of blood lipids in rats in each group:CHOL in model group were higher than that in normal group(P<0.05);TG in the model group,Tangshen’an low,medium dose group was higher than the normal group(P<0.01,P<0.05).TG in each treatment group was lower than that in model group(P<0.01,P<0.05).LDL-C in model group,Tangshen’an low-dose group and western medicine group were significantly higher than those in normal group(P<0.05),LDL-C in model group were higher than that in Tang shen’an high-dose group(P<0.05).4.Changes of renal function in rats in each group:The BUN in model group and western medicine group was higher than that in normal group(P<0.01,P <0.05).The BUN in middle and high dose Tangshen’an group was lower than that in model group(P<0.01,P<0.05).The Scr in the model group and the low dose of Tangshen’an group were significantly higher than that in the normal group(P<0.01).The Scr in middle and high dose Tangshen’an group and western medicine group was lower than that in the model group(P<0.01,P<0.05);.5.Changes of 24-hour urine microalbumin in rats in each group:24h urinary album inin the model group and each treatment group was higher than that in the normal group(P<0.01).6.Changes of renal tissue Nrf2 in rats in each group:The expression of Nrf2 protein in renal tissue of model group and each treatment group was higher than that of normal group(P<0.01),and the expression of Nrf2 protein in middle and high dose group and western medicine group was higher than that in model group(P<0.01).The expression of Nrf2 protein in renal tissue of the low dose of Tangshen’an group was lower than that of western medicine group(P<0.01).7.Changes of renal tissue HO-1 in rats in each group:The expression of HO-1 in model group and each treatment group was higher than that in normal group(P<0.01)andthe expression of HO-1protein in middle and high dose group and western medicine group was higher than that in model group(P<0.01).The expression of HO-1 in the low dose Tangshen’an group was lower than that in the western medicine group(P<0.01)and theexpression of HO-1in high dose Tangshen’an group was higher than that in western medicine group(P<0.05).8.The expression of renal tissue NF-κBp65 in rats in each group:8.1Western blot was used to detect the expression of NF-κBp65 protein in rat kidney:The expression of NF-κBp65 in renal tissue of model group and each treatment group was higher than that of normal group(P<0.01,P<0.05).The protein expression of NF-κBp65 in renal tissue of each treatment group was lower than that in model group(P<0.01).The expression of NF-κBp65 in renal tissue of low dose Tang shen’an group was higher than that in western medicine group(P <0.01).8.2The expression of NF-κBp65m RNA in rat kidney tissue was detected by RTPCR:The expression of NF-κBp65 m RNA in renal tissue of model group,low and middle dose Tangshen’an group and western medicine group were higher than that in normal group(P<0.01).The expression of NF-κBp65 m RNA in kidney of low dose Tangshen’an group was higher than that in western medicine group(P<0.01).Conclusion:1.Tangshen an decoction can reduce fasting blood glucose,24h urinary albumin,regulate blood lipids,protect renal function in diabetic rats.2.Tangshen’an decoction activates Nrf2/ARE signaling pathway,up-regulates the expression of the antioxidant enzyme gene HO-1,inhibits the expression of NF-κBp65 m RNA and protein,improves the renal inflammatory response and exerts the anti-oxidativeeffect. |
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