Study On The Combined Disease And Syndrome Model Of EAT Rats With Liver Depression And Spleen Deficiency

Purpose:To establish a model of combined disease and syndrome in rats with liver stagnation and spleen deficiency,and to explore the evaluation criteria of the syndrome model of liver stagnation and spleen deficiency in EAT rats.?Material and method:Forty female Sprague-Dawley rats were randomly divided into 4groups according to their body weight: 10 in the normal group,10 in the model group,10 in the Chinese medicine treatment group,and 10 in the falsification group.The normal group was not modeled,and only rats were given distilled water and conventional feed during the modeling period.The model group,the Chinese medicine treatment group and the falsification group were fed with high-iodine water at a concentration of 500 mg/L.On the22 nd day,the thyroglobulin(PTg)and the complete Freund's adjuvant were mixed in an equal volume and fully emulsified and injected into the rat.The double hind paws were subcutaneously,and the second time after 2 days,it was the first immunization.On the 36 th day,the PTg was fully emulsified with an equal volume of incomplete Freund's adjuvant and injected into the back of the rat.Three times on the 36 th,43d,and 50 d,as a booster.The 56 th day ended the disease modeling.Three times on the 36 th,43d,and 50 d,as a booster.The 56 th day ended the disease modeling.At the same time,the EAT disease model was established,and the TCM model of liver stagnation and spleen deficiency was established.On the 29 th day,the rats in the model group,the Chinese medicine treatment group and the falsification group were respectively subjected to swimming + diet restriction + activity restriction.After the liver stagnation and spleen deficiency EAT syndrome model was successfully established,it entered the treatment stage,that is,on the 58 th day,the rats in the normal group and the model group were given normal saline;the Chinese medicine treatment group was given the soft Jianxiaoxiao soup;the syndrome group was given Jinqi Shenqi Pills aqueous solution was administered.Continuous treatment for 56 d.At the end of the treatment,the TCM syndrome scores,thyroid weight index,thyroglobulin antibody,thyroid peroxidase antibody,IL-1,and TNF-? were compared.Results:1.According to the "Practical TCM Syndrome Animal Model",the symptom scores of rats with liver stagnation and spleen deficiency syndrome were evaluated: compared with the normal group,the quantitative scores of TCM symptom syndrome(mental state,arched back,stool,etc.)in the model group were higher than those in the normal group(P < 0.05).It is suggested that the model group rats have successfully replicated the model of liver stagnation and spleen deficiency syndrome.2.Compared with the normal group,the TGAb and TPOAb in the model group were higher than those in the normal group(P<0.05),and the thyroid weight index was higher than the normal group.The model group rats were prompted to successfully replicate the EAT model.3.Compared with the normal group,the levels of TNF-? and IL-1 in the model group were higher than those in the normal group(P<0.05).Compared with the model group,the TNF-?and IL-1 in the Chinese medicine treatment group and the syndrome group were lower than the model group.(P<0.05).It was suggested that the model group rats successfully replicated the EAT model of liver stagnation and spleen deficiency.Conclusion:1.High-iodine water feeding combined with porcine thyroglobulin immune booster injection,and simultaneous "chronic restraint stress + excessive fatigue + eating dislocation method" can replicate the EAT liver stagnation and spleen deficiency syndrome model.2.The evaluation of EAT liver stagnation and spleen deficiency syndrome model can be used to evaluate the success of animal models through TCM symptom scores,thyroid antibodies,immune cytokines and drug counter-evidence methods.