| Purpose:To study the effect of Chinese herbal compound Pidan Ning on insulin resistance and insulin receptor substrate 1 tyrosine phosphorylation in skeletal muscle cells of diabetic rats.Materials and methods: 100 SPF-level Wistar male rats aged 60-70 days were randomly selected by weight to select 20 normal control groups,and the remaining 80 were used as glucose-damaged damaged modules,fed with basic diet and high sugar.In the fat diet,the rats were free to eat water,the relative humidity was maintained at 30%-70%,and the room temperature was controlled at 18-20 °C.After 12 weeks of feeding,the rats in the model group were tested for glucose tolerance test(OGTT).After confirming the successful model of glucose regulation,the model rats that reached the standard of glucose regulation impairment were randomly divided into three times.For the model group,the spleen treatment group and the metformin treatment group.Six rats in each group were randomly selected for body weight,fasting blood glucose(FBG)and OGTT 2h blood glucose measurement,and insulin resistance was evaluated by glucose infusion rate(GIR)in normal blood glucose-high insulin clamp technique.In the case,rats were sacrificed by excessive anesthesia.The remaining rats in each group were distilled water of 5ml·kg-1·d-1,distilled water of 5ml·kg-1·d-1,10g·kg-1·d-1 spleen decoction,150mg·kg-1·d-1 metformin suspension was administered orally for 4 weeks,and the average daily dose was 1 time.After treatment,6 rats in each group were randomly selected.The same operation was performed,and the rats were sacrificed.Rat hindlimb skeletal muscle,frozen at-70 ° C,Western blot analysis of tyrosine phosphorylation of IRS-1 protein expression in skeletal muscle cells,and analysis of IRS-1 tyrosine phosphorylation The correlation between levels and insulin resistance.At the end of the experiment,all rats were sacrificed.Results:1.The evaluation of insulin resistance in experimental rats: before the drug intervention,the glucose infusion rate of the model control group,the spleen Yining group and the metformin treatment group were significantly lower than the normal control group(P<0.01),and the GIR between the three groups.There was no significant difference(P>0.05).After drug intervention,the GIR of the spleen and the metformin group were significantly lower than that of the normal control group(P<0.01),but they were significantly higher than the model control group(P<0.01).),and there was no significant difference in GIR between the two groups(P>0.05)2.Evaluation of the degree of tyrosine phosphorylation of insulin receptor substrate 1 in skeletal muscle cells: After drug intervention,the degree of IRS-1 tyrosine phosphorylation in skeletal muscle cells of spleen-nanning group and metformin group was lower than that of normal control group(P<0.01 or 0.05),but it was significantly higher than model control group(P<0.01 or 0.05).There was no significant difference between the spleen treatment group and the metformin treatment group(P>0.05).3.The correlation between insulin resistance and the degree of receptor substrate 1 tyrosine phosphorylation in skeletal muscle cells.The degree of tyrosine phosphorylation of receptor substrate 1 in skeletal muscle was significantly positively correlated with glucose infusion rate(r =0.762).Conclusion:1.The Pidan Ning can obviously improve the insulin resistance of the rats with impaired glucose regulation.2.Pidan Ning can significantly increase the degree of receptor substrate 1 tyrosine phosphorylation in skeletal muscle cells of rats with impaired glucose regulation.3.Pidan Ning can improve insulin resistance in rats with impaired glucose regulation by increasing the regulation of IRS-1 tyrosine phosphorylation in skeletal muscle cells of rats with impaired glucose regulation. |
PDF Full Text Request