Sulfation Modification And Biological Activity Of Hericium Erinaceus Polysaccharide

This topic with Hericium erinaceus as materials,water extract-alcohol precipitation through extract Hericium erinaceus polysaccharide?Hericium esrinaceus mixture polysaccharide,HEPM?,chlorosulfonic acid-pyridine method sulphation modification to obtain Hericium erinaceus polysaccharide sulfate?Hericium esrinaceus sulfate polysaccharide mixture,S-HEPM?.For its separation and purified,determine the degree of substitution of the sulfate group,the glycosidic bonds,components and monosaccharide composition of Hericium erinaceus sulfated polysacchardes were analyzed.The antitumor,antioxidant and immumomodulatory activities of Hericium erinaceus polysaccharides before and after modification were studied.The test results are as follows:The response surface method used optimize the optimize conditions for optimal sulfating modification of Hericium erinaceus polysaccharide:the molar ratio of chlorosulfonic acid-pyridine is 1:4,the reaction temperature is 59?,the reaction time is 2.6 h,the substitution degree of sulfate group is 0.457.S-HEP is further purified by ion exchange chromatography column and gel fitration chromatography column,obtained homogeneous Hericium esrinaceus sulfated polysaccharides?Hericium esrinaceus sulfated polysaccharides,S-HEP?.The total sugar content was 89.98%±0.23%by phenol-sulfuric acid method,no protein was detected by Bradford method,uronic acid was 0.53%±0.12%by carbazole-sulfuric acid method.Infrared spectrum display:at 1270 cm^-1and 834 cm^-1,the stretching vibration of S=O and C-O-S bond is increased respectively,this indicates the sulfuric acid group replaces the hydrogen on the hydroxyl group in the hericium polysaccharide,the sulfuric acid group combines with hericium polysaccharide to form esters.The monosaccharide composition of S-HEP was Glc,Man and Gal,composition ratio of monosaccharide was 1.47:0.21:1.00.Results of antitumor test showed:under the same conditions,S-HEP has better inhibitory effect on human gastric cancer cell SGC-7901;at concentration of 100?g/m L,S-HEP showed extremely significant difference;at 48 h,the IC₅₀of HEP and S-HEP wes 425.09±2.49?g/m L and 308.36±8.74?g/m L,respectively;at same concentration,the number of cells decreased under action of S-HEP,presenting vacuolization,and the floating cells increased;S-HEP can better promote the enzyme activities of Caspase-3,8,9,leading to rapid apoptosis of SGC-7901 cells.At same concentration,S-HEP was more effective than HEP in inhibiting migration of SGC-7901 cells.Results of immumomodulatory test showed:under same conditions,S-HEP has stronger growth ability on RAW264.7 cells;at concentration of 100?g/m L,showed significant difference;at concentration of 200?g/m L,showed extremely significant difference.At same concentration,S-HEP significantly increased the phagocytic capacity of RAW264.7 cells compared of HEP,enhance levels of nitric oxide?NO?and cytokines?IL-6,IL-1??,thus promoting immune regulation of S-HEP.Results of anti-oxidant test showed:EC₅₀value of S-HEP,caculated by the scavenging rate of DPPH,ABTS,·OH and·O^2-,is 125.416±1.87?g/m L,146.73±2.05?g/m L,127.42±1.97?g/m L and 114.39±1.89?g/m L,and EC₅₀value of HEP is respectively 222.76±2.11?g/m L,312.85±2.40?g/m L,383.78±2.45?g/m L and399.24±2.45?g/m L.The total antiocidant value of S-HEP and HEP is respectively169.91±2.04?g/m L and 243.15±2.25?g/m L,it indicates that S-HEP has good antioxidant capacity.The sulfated polysaccharides in hericium bacteria has good activity in anti-tumor,immumomodulatory,anti-oxidation and many other aspects,it can be widely used as a leading active compound in drug and clinical application of cancer prevention and treatment,it can provide scientific basis for the study of polysaccharides from hericium bacteria.