| Objective:To establish a animal model of diabetic ulcer that mimics the mechanism of diabetic skin ulcer as far as possible.Methods:High-sugar and high-fat diet combined with the low-dose intraperitoneal injection streptozotocin to establish rat model of diabetic.Observing the morphology of diabetic rats and comparing the changes of blood sugar and body weight before and after modeling.Then a square(2x2cm)ulcer wound was made on the back of experimental diabetic rats.Observing the morphology of ulcer wounds in diabetic rats.Histopathological changes of ulcer wounds were observed by histological examination.t test for comparison between two groups.Using P=0.05 as the significance test standard.P<0.05 represents statistically significant dif-ferences between group.Results:(1)After the establishment of diabetic rat model,the morphological observation showed that obvious symptoms of polyuria,Polydipsia and weight loss.(2)When the changes of blood sugar and body weight were compared before and after modeling,the body weight after modeling(288.5862±62.29687)was lower than before modeling(302.8815±65.06324)(P<0.05),the blood sugar after modeling(24.9785±3.95085)was significantly higher than before modeling(5.5400±0.79310)(P<0.05).(3)Ulcer-like changes were observed in the morphology and histomorlphology of skin ulcers in diabetic rats.Conclusion:An ideal and stable animal model of diabetic ulcer was established through this study.Objective:To obtain the optimal concentration of exogenous stromal cell-derived factor-1 on the healing of ulcers in diabetic rats.Methods:Diabetie ulcer models were established by SD male rats according to the method of the first part.These models were randomly divided into control-group(8 rats)and three SDF-1 treatment groups(24 rats,and were divided into high-does group,medium-does group and low-does group with 8 rats in each group).The ulcer wounds of each groups were treated with 0.9%saline,50 ug/kg,25 ug/kg and 12.5 ug/kg SDF-1 respectively.The ulcer area was recorded with the method of hyaline membrane tracing at 3,7,14,21days post-injury.The growth and inflammation of granulation tissue were macroscropically observed.The fibroblasts and capillaries in the granulation tissue were observed under an electron microscopy at 21 days.Enzyme linked immunosorbent assay was used to detect the expression of fibroblast growth factor,fydroxyproline,vascular endothelial growth factor and stromalcell derived factor-1 in ulcer tissue.Variance analysis was used between groups,and LSD-t for comparison between two groups.Using P=0.05 as the significance test standard.P<0.05 represents statistically significant differences between group.Results:(1)Macroscopic observation of ulcer wounds showed that the granulation tissue formation in the middle-dose group was better than the other three groups,and the peripheral inflammation was light.(2)Histological examination showed that fibroblasts,capillaries and inflammatory cell were observed in the ulcer wounds of four groups.However,the number and patterm of fibroblasts and capillari esin medium-does group were better than those in the other three groups.(3)When the wound healing rate(%)were compared in four groups at different time,medium-does group(46.43±18.14,67.01±15.14,80.40±4.37,86.96±2.62)was better than control-group(29.73±8.88,42.95±8.51,69.95±4.99,78.43±6.26)and low-does group(22.78±16.00,57.46±10.84,68.28±10.06,77.29±11.17)(P<0.05);There was no statistically significant difference among the other groups(P>0.05).(4)When the FGF(ng/L)were compared in four groups at different time,medium-does group(8.10±1.17,13.98±4.35,15.42±1.68,18.31±0.70)was better than control-group(11.25±1.96,12.87±1.53,12.23±2.83,10.72±2.93),high-group(9.90±2.69,9.52±1.13,12.20±2.13,l 4.06±3.12)and low-group(9.23±0.55,11.24±2.82,11.22±3.04,12.55±2.10)(P<0.05);There was no statistically significant difference among the other groups(P>0.05).(5)When the HYP(ug/L)were compared in four groups at different time,medium-does group(257.26±33.20,280.55±45.90,342.89±38.96,403.63±40.00)was better than control-group(236.96±52.42,244.35±93.03,313.54±71.70,308.80±80.21),high-group(210.97±28.38,208.74±33.59,218.27±36.32,209.00±45.00)and low-group(222.20±79.38,253.58±70.35,289.84±99.20,276.56±78.03)(P<0.05);The control-group>high-group,low-group>high-group(P<0.05);There was no significant difference between the control-group and the low-dose group(P>0.05).(6)When the VEGF(ng/L)were compared in four groups at different time,medium-does group(76.84±12.86,48.01±12.70,101.95±8.81,121.53±15.11)was better than control-group(53.59±21.20,61.41±16.05,73.98±11.15,115.95±41.37),high-group(73.97±7.09,71.52±9.88,73.19±7.22,86,03±10.75)and low-group(63.39±15.73,68.03±25.81,70.06±7.29,64.62±17.96)(P<0.05);The control-group>low-group,high-group>low-group(P<0.05);There was no significant difference between the control-group and the high-dose group(P>0.05).(7)When the SDF-l(pg/ml)were compared in four groups after SDF-lintervention at different time,medium-does group(271.40±112.72,389.80±174.63,352.47±41.00,394.28±34.63)was equivalent to control-group(262.12±38.150,205.82±83.65,190.44±54.82,397.05±95.57)(P<0.05);There was no statistically significant difference among the other groups(P>0.05).(8)Comparison of the rate of cell factors'changes in each group:FGF(ng/L):compared with the control-group,middle-dose group>high-dose group>low-dose group(P<0.05);compared with the middle-dose group,high-dose group>low-dose group>control-group(P<0.05).HYP(ug/L):middle-dose group>high-dose group(P<0.05),there was no statistically significantdif ference among the other groups(P>0.05).VEGF(ng/L):control group>low dose group(P<0.05),middle dose group>low dose group(P<0.05),the other groups weren ot statistically significant.SDF-1(pg/ml):There was no statistically difference in the rate of change of SDF-1 between the groups(P>0.05).Conclusion:SDF-1 at appropriate concentrations(25ug/kg)can significantly promote the healing of skin ulcers in diabetic rats,which can provides a new therapeutic method for improving the healing rate and quality of tissue injury such as diabetic ulcer. |
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