An Experimental Study On The Safety Of Five Massage Methods In DVT Rats

ObjectiveUsing five manipulation methods to qualitatively and quantitatively intervene in deep vein thrombosis(DVT)rats,to study the effect of massage five methods on deep vein thrombosis,and to evaluate the safety of five massage methods on deep vein thrombosis Provide theoretical basis for clinical diagnosis and treatment.MethodsUsing the massage manipulation simulator to simulate the five methods of point method,dial method,kneading method,pushing method and pulling method,the qualitative and quantitative intervention of the deep vein thrombosis rat model was carried out,and the following three aspects were used to study the effects of massage on thrombosis influences:1 Ultrasound elasticity evaluation of DVT rats with five massage methods.Rats in each group were intervened for 3 days and 10 days later,using digital color Doppler ultrasound diagnostic apparatus to conduct ultrasound elastography on the rats,observe the changes and calculate the strain rate ratio.2.Effect of five massage methods on blood coagulation and fibrinolysis in DVT rats.Rats in each group were taken for intervention for 3 days and 10 days later.The automatic coagulation analyzer was used to detect the expression changes of PT,APTT,TT,and FIB,and the DD method was used to detect D-Dimer,TXB2,6-Keto-The expression of PGF1αchanges.3.The effect of five massage methods on the hemorheology of DVT rats.Rats in each group were taken for intervention for 3 days and 10 days later,and the blood samples were collected by automatic hemorheology analyzer to detect the changes of low-cut,medium-cut,high-cut and plasma viscosity of DVT rats.From the three aspects of ultrasound,blood coagulation,fibrinolysis,and blood rheology,the comprehensive analysis of the effect of the five massage methods on deep vein thrombosis and its safety was evaluated.Result1 Ultrasound elasticity evaluation of five methods of massage on DVT rats1.1 Ultrasound elastography:After 3 days of intervention,there was no difference between the point method,dial method,kneading method,pushing method,and pulling method group compared with the model group(P>0.05).After 10 days of intervention:there was no difference between the point method,dial method,kneading method,pushing method,pulling method group and model group(P>0.05).1.2 Strain rate ratio:After 3 days of intervention,the point method,push method,pulling method group and the model group had a lower ratio(P<0.05),and the dialing method,kneading method and the model group had no difference(P>0.05).After 10 days of intervention:the point method,dial method,kneading method,pushing method,pulling method group were all different from the model group(P>0.05).2.Effect of five massage methods on blood coagulation and fibrinolysis in DVT rats2.1 Prothrombin time(PT):After 3 days of intervention,there was no difference between the point method,dial method,kneading method,pushing method,and pulling method group compared with the model group(P>0.05).After 10 days of intervention:the time of the dial method group and the model group was shorter(P<0.05),and there was no difference between the point method,kneading method,pushing method,and pulling method group and the model group(P>0.05).2.2 Activated partial thromboplastin time(APTT):After 3 days of intervention,the time of the kneading group was shorter than that of the model group(P<0.05),and there was no comparison between the point method,dial method,pushing method,and pulling method group compared with the model group.Difference(P>0.05).After 10 days of intervention:the time between the dial method group and the model group was significantly shorter(P<0.01),and there was no difference between the point method,kneading method,pushing method,and pulling method group compared with the model group(P>0.05).2.3 Thrombin time(TT):After 3 days of intervention,there was no difference between the point method,dial method,kneading method,pushing method and pulling method compared with the model group(P>0.05).After 10 days of intervention:there is no difference between the point method,dial method,kneading method,pushing method,pulling method and model group(P>0.05).2.4 Fibrinogen(FIB):After 3 days of intervention,there was no difference between the point method,dial method,kneading method,pushing method and pulling method compared with the model group(P>0.05).After 10 days of intervention:there is no difference between the point method,dial method,kneading method,pushing method,pulling method and model group(P>0.05).2.5 D-dimer:After 3 days of intervention,the content of the dial method group was higher than that of the model group(P<0.05),and there was no difference between the point method,kneading method,pushing method and pulling method group compared with the model group(P>0.05).After 10 days of intervention:there was no difference between the point method,dial method,kneading method,pushing method,pulling method group and model group(P>0.05).2.6 Thromboxane B2(TXB2):After 3 days of intervention,there was no difference between the point method,dial method,kneading method,pushing method and pulling method group compared with the model group(P>0.05).After 10 days of intervention:the content of the point method group and the model group increased(P<0.05),and there was no difference between the dial method,kneading method,pushing method,and pulling method group and the model group(P>0.05).2.7 6-ketoprostaglandin F1α(6-Keto-PGF1α):After 3 days of intervention,the content of dial method,push method and model group increased(P<0.05),and the content of pull method and model group was significant(P<0.01)There was no difference between the point method and the kneading method compared with the model group(P>0.05).After 10 days of intervention:the content of the point method,push method and pulling method group was significantly reduced compared with the model group(P<0.01),the content of the kneading method group and the model group was reduced(P<0.05),although the dial method group was compared with the model group The content was reduced but there was no statistical difference(P>0.05).3 E ffect of five massage methods on blood rheology of DVT rats3.1 Low shear rate:After 3 days of intervention,the push rate,the pulling method and the model group reduced the shear rate(P<0.05),and the dialing method and the rubbing method group had no difference compared with the model group(P>0.05).After 10 days of intervention:there is no difference between the point method,dial method,kneading method,pushing method,pulling method and the model group(P>0.05).3.2 Intermediate shear rate:After 3 days of intervention,there was no difference between the sham operation,point method,dial method,kneading method,pushing method,pulling method and the model group(P>0.05).After 10 days of intervention:the point method,dial method,kneading method,pushing method,pulling method were not different from the model group(P>0.05).3.3 High shear rate:After 3 days of intervention,there was no difference between the point method,dial method,kneading method,pushing method and pulling method compared with the model group(P>0.05).After 10 days of intervention:the point method,dial method,kneading method,push method compared with the model group,the shear rate decreased(P<0.05),the pull method group and the model group had no difference(P>0.05).3.4 Plasma viscosity:After 3 days of intervention,the shear rate of the dial method,kneading method group and model group decreased(P<0.05),and there was no difference between the point method,push method and pulling method compared with the model group(P>0.05).After 10 days of intervention:point method,dial method,kneading method,pushing method,pulling method were not different from the model group(P>0.05).Conclusion1 Ultrasound elasticity shows that there are no risks for DVT rats with five massage methods.2 Blood coagulation and fibrinolysis showed that after 3 days of intervention with massage,the method of spotting,pushing and pulling on DVT rats is highly safe,but after 10 days of intervention,there are certain risks in pointing,pushing and pulling;3 days,10 days at two time points,dial method,rubbing method on DVT rats are risky.3 Hemorrheology showed that there are no risks for DVT rats with five massage methods.The five-point massage method,point method,and pulling method are highly safe for early intervention of DVT rats,but as the number of intervention days increases,point method,pushing method,and pulling method also have certain risks;dialing method and kneading method There is a risk of acting on DVT rats.It is recommended that the treatment of deep venous thrombosis in the clinical use of soft methods such as point method and pushing method,and the dial method and the rubbing method be used with caution.The five methods of massage on DVT rats’ time selection and strength still need further study.