| ObjectiveTo observe(1)Effects of electroacupuncture(EA)on behavior and expressions of glial fibrillary acidic protein(GFAP)and nerve grow factor(NGF)in ischemic cortex and hippocampus of rats with cerebral ischemia.(2)Effects of EA on blood lipid,behavior,brain histomorphology and expression of GFAP and NGF in ischemic cortex and CA1?CA2?CA3?dentate gyrus(DG)area in hippocampus of rats with hyperlipemia middle artery thrombus.MethodsExperiment 124 male SD rats were randomly divided into sham operation group,model group,EA1 group and EA2 group.Rats fed with common diet for 7 days,and used 50%FeC13 to induce and establish cerebral ischemia model on the 8th day.EA1 group stabbed in Fenglong(ST 40)once a day for 7 days before the model was established and stabbed in ST 40 and Baihui(GV 20)once a day for 7 days after the model was established.EA2 group stabbed in ST 40 and GV 20 once a day for 7 days after the model was established.After 1 day of modeling,all rats were scored for neurological function.Immunohistochemical method was used to detect the expression of GFAP and NGF in ischemic cortex and hippocampal CA1,CA2,CA3,DG area.Experiment 249 male SD rats were randomly divided into normal group,sham operation group,model group,EA1 group and EA2 group.Rats in normal group were fed with common diet for 63 days.Rats in sham group were fed with high-fat diet(HFD)for 63 days.Rats in model group and EA group were fed with HFD for 49 days,and used 50%FeCl3 to induce and establish hyperlipemia middle artery thrombus on the 50th day.EA1 group stabbed in ST 40 once a day for 7 days before the model was established,and stabbed in ST 40 and GV 20 once a day for 14 days after the model was established.EA2 group stabbed in ST 40 and GV 20 once a day for 14 days after the model was established.After 1 day of modeling,all rats were scored for neurological function.The levels of total cholesterol(TC),triglyceride(TG),high density lipoprotein(HDL)and low-density lipoprotein(LDL)in serum were detected on the 1st,7th and 14th day after operation.The morphological changes of neuron cells were observed by hematoxylin-eosin(HE).Immunohistochemical method was used to detect the expression of GFAP and NGF in ischemic cortex and hippocampal CA1,CA2,CA3,DG area.ResultsExperiment 1:(1)Neuroethology:In model group,the rats showed the symptoms of nerve defect turning to the opposite side,and NDS was higher.Compared with model group,in EA group,the nerve defect symptom and NDS reduced significantly(P<0.05).(2)Immunohistochemical results:In the ischemic penumbra:In model group,the AOD of GFAP generally increased significantly(P<0.05)at the 1 st and 7th day after operation.Compared with model group,in EA1 group,the AOD of GFAP showed an increasing trend at the 7th day after operation.In EA2 group,the AOD of GFAP increased significantly(P<0.05)at the 1 st day after operation.In hippocampal CA1 region:In model group,the AOD of GFAP generally increased significantly(P<0.01)at the 1 st and 7th day after operation.Compared model group,in EA1 and EA2 group,the AOD of GFAP decreased significantly(P<0.05)at the 1st and 7th day after operation.In hippocampal CA2 region:In model group,the AOD of GFAP generally increased significantly(P<0.01)at the 1st,7th day after operation.Compared with model group,in EA1 group,the AOD of GFAP decreased significantly(P<0.05)at the 1st and 7th day after operation.In EA2 group,the AOD of GFAP showed a decreasing trend at the 1st,7th day after operation.In hippocampal CA3 region:In model group,the AOD of GFAP generally increased significantly(P<0.01)at the 1 st and 7th day after operation.Compared with model group,in EA1 and EA2 group,the AOD of GFAP decreased significantly(P<0.01)at the 1st and 7th day after operation.In hippocampal DG region:In model group,the average optical density(AOD)of GFAP generally increased significantly(P<0.01)at the 1st and 7th day after operation.Compared with model group,in EA1 group,the AOD of GFAP decreased significantly(P<0.05)at the 1st and 7th day after operation.In EA2 group,the AOD of GFAP decreased significantly(P<0.05)at the 7th day after operation.In hippocampal CA1 region:In model group,the AOD of NGF generally increased significantly(P<0.01)at the 1st and 7th day after operation.Compared with model group,in EA1 group,the AOD of NGF increased significantly(P<0.01)at the 7th day after operation.In EA2 group,the AOD of NGF increased significantly(P<0.01)at the 1st,7th day after operation.Experiment 2:(1)Blood lipids:In model group,TC and LDL increased significantly(P<0.05)at the 1st,7th,14th day after operation,HDL decreased significantly(P<0.05)at the 7th day after operation,,TG increased significantly(P<0.05)at the 14th day after operation,which indicating that hyperlipidemia model was successfully established and stable.Compared with model group,in EA1 group,TC decreased significantly(P<0.05)at the 1 st day after operation,LDL decreased significantly(P<0.05)at the 7th day after operation,LDL and TG decreased significantly(P<0.05)at the 14th day after operation.In EA2 group,TC decreased significantly(P<0.05)at the 1st day after operation,TG and LDL decreased significantly(P<0.05)at the 14th day after operation.This indicates that EA intervention may have the effect of reducing serum lipid level in rats.The degree of reduction in EA1 group is higher than that in EA2 group,which indicates that EA pretreatment has better effect of reducing blood lipid.(2)Neuroethology:The rats in model group showed nerve defect symptoms such as contralateral upper limb weakness and turning around to the contralateral.The NDS was higher.Compared with model group,EA1 group and EA2 group showed significantly decreased in neurological deficit symptoms and NDS(P<0.05).(3)Brain tissue morphology:The ischemic penumbra:In normal group,neurons have complete morphology and normal overall tissue morphology.In model group,the neuron cell body was reduced,the nucleus was solidified or dissolved,the intercellular space was enlarged,and the cell arrangement was disordered.In EA group,neuron morphology is close to normal,some abnormal neuronal cells can be seen,and overall tissue morphology is close to normal.Hippocampal CA1,CA2,CA3 and DG regions:In normal group,neurons are arranged in order,with clear layers and complete morphology.In model group,the cells were irregularly arranged and some cells were scattered.Neurons in EA group are relatively orderly arranged and relatively complete in shape.(4)Immunohistochemical results:In the ischemic penumbra:In model group,the average optical density(AOD)of GFAP generally increased significantly(P<0.01)at the 1st,7th and 14th day after operation.Compared with model group,in EA1 group,the AOD of GFAP increased significantly(P<0.05)at the 7th day after operation and decreased significantly(P<0.05)at the 14th day after operation;in EA2 group,the AOD of GFAP decreased significantly(P<0.05)at the 1th and 14th day after operation.In hippocampal CA1 region:In model group,the AOD of GFAP generally increased significantly(P<0.05)at the 1 st,7th day after operation.Compared with model group,in EA1 and EA2 group,the AOD of GFAP generally decreased significantly(P<0.05)at the 1 st,7th and 14th day after operation.In hippocampus CA2 region:In model group,the AOD of GFAP generally increased significantly(P<0.05)at the lst,7th,14th day after operation.Compared with model group,in EA1 and EA2 group,the AOD of GFAP showed an increasing trend at the 7th day after operation,and decrease significantly(P<0.05)at the 14th day after operation.In hippocampal CA3 region:In model group,the AOD of GFAP generally increased significantly(P<0.01)at the 1st,7th,14th day after operation.Compared with model group,in EA1 group,the AOD of GFAP increased significantly(P<0.05)at the 7th day after operation and decreased significantly(P<0.05)at the 14th day after operation.In EA2 group,the AOD of GFAP showed an increasing trend at the 7th day after operation and a decreasing trend at the 14th day after operation.In hippocampus DG region:In model group,the AOD of GFAP increased significantly(P<0,05)at the 1st,14th day after operation.Compared with model group,in EA1 group,the AOD of GFAP increased significantly(P<0.01)at the 7th day after operation,and showed a decreasing trend on the 14th day after operation.In EA2 group,the AOD of GFAP increased significantly(P<0.05)at the 7th day after operation.In hippocampal CA1 region:In model group,the AOD of NGF showed an increasing trend at the 1st,7th,14th day after operation.Compared with model group,in EA1 group,the AOD of NGF increased significantly(P<0.05)at the 1st,7th,14th day after operation.In EA2 group,the AOD of NGF increased significantly(P<0.05)at the 1st day after operation and showed an increasing trend at the 7th,14th day after operation.ConclusionEA on ST 40 before cerebral ischemia and EA on ST 40 and GV 20 after cerebral ischemia.which can reduce the neurological deficit function of cerebral ischemia rat,reduce the neurological function score,regulate the expression of GFAP in ischemic penumbra and CA1,CA2,CA3 and DG regions of hippocampus of rats,and the expression of NGF protein in hippocampal CA1 region,regulate the activation expression of astrocytes,and restore damaged nervous system.EA on ST 40 at the stage of hyperlipemia and EA on ST 40 and GV 20 after cerebral ischemia,which can regulate the metabolism of blood lipids in rat serum,reduce the neurological deficit function of cerebral ischemia rats,promote the recovery of brain tissue and neuronal cell morphology,regulate the expression of GFAP in ischemic penumbra and CA1,CA2,CA3 and DG regions of hippocampus of rats,and the expression of NGF protein in hippocampal CA1 region. |
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