| The main objective was to combine the indispensable amino acid requirements, determined using indicator amino acid oxidation, into a single total parenteral nutrition solution, the B/P profile, and compare the performance of parenterally-fed neonatal piglets receiving the B/P profile to piglets receiving a different parenteral amino acid pattern. The first experiment demonstrated that the B/P profile contained at least one severely limiting amino acid. Consequently, lysine, leucine, methionine, threonine and/or proline were added to the B/P profile and 14C phenylalanine oxidation was measured to determine which of these amino acids was first limiting. Plasma lysine concentrations increased significantly (p<0.05) when the B/P pattern was supplemented with lysine. In combination with previous research, it was determined that proline may be indispensable to the parenterally-fed piglet. No other amino acid supplementation significantly (p>0.05) reduced oxidation. Therefore, we were unable conclude if any of these amino acids were limiting in the B/P profile. |
