| The goal of the project was to develop a nanoparticle-based carrier for possible drug targeting and gene delivery.; A two-step desolvation process was used to synthesize nanoparticles from gelatin A and B. The important synthesize parameters were investigated. The DNA binding capacity of gelatin A and B nanoparticles was conformed by agarose gel electrophoresis. COS-1, 143B, HELA and 293 cells were used for the gene transfection studies in vitro. Lac Z was used as a reporter gene for detection of transfection.; Controlling the experimental conditions, nanoparticles with defined size ranges and narrow size distribution can be synthesized. Gelatin A nanoparticles have a higher binding capacity compared to gelatin B nanoparticles. Therefore gelatin A nanoparticles were selected as vector for gene transfection studies. Gelatin A nanoparticles showed the ability to deliver the Lac Z gene to COS-1 and 143B cells. |
