| Due to the advantages of nanomaterials,such as small size,large specific surface area,special structure and high reactivity,nanomaterials related to biomedicine have been widely reported.Nanomaterials with various morphologies have been exploited,and great progress has been made in biomedical detection,imaging and therapy.In the past ten years,nano metal-organic frameworks（NMOFs）and polydopamine nanoparticles（PDA NPs）as excellent materials in polymer Nanomaterials have shown great advantages in biomedical detection,imaging and therapy.In this thesis,we mainly studied the application of NMOFs and PDA NPs in biomedical detection and cancer therapy.Specifically divided into the following two parts:（1）In the first part of this thesis,we successfully exploited a porphyrin-based NMOF PCN-224 as a sensitive ratiometric fluorescent probe for phosphate detection based on the dual emission fluorescence of the H2Tcpp ligand.In addition,we verified that based on the linker-elimination strategy,synthesized PCN-224 with six-connected Zr O clusters can effectively improve the detection sensitivity.The detection limit of the nanoprobe is 54 n M.Finally,we successfully measured phosphate in human serum and drinking water by this nanoprobe.（2）In the second part of this thesis,we formulated an efficient 808 nm near-infrared laser triggered NO release nanoplatform PDA-SNO,in which PDA nanoparticles were used to conjugate with a type of heat-unstable NO donor S-nitrosothiol（SNO）.On the one hand,upon 808nm near-infrared laser irradiation,the PDA nanoparticle as a heat source,can significantly increase the local temperature in cancer cells and kill them.On the other hand,the heat generated by PDA of PDA-SNO nanoparticles can be used to trigger NO donor to release NO.MTT and cell imaging experiments confirmed the NO can be controlled release from PDA-SNO nanoplatform,and the cancer cells can be effectively killed by PDA-SNO nanoplatform through gas-photothermal synergistic therapy. |
