Research And Development Of Mastiche Toothpaste Products

PurposeOral diseases have become a major disease that affects people's health and quality of life.As one of the important means to prevent oral diseases,functional toothpaste has attracted widespread attention.The use of modern scientific extraction technology to apply natural Chinese herbal medicine extracts to toothpaste products,so that it can not only achieve the purpose of cleaning the oral cavity,but also have the functions of disease prevention and oral health care.It is a feature of the research and development of functional toothpastes in my country and has a good market prospect and development potential.However,due to the lack of systematic research on the efficacy of Chinese herbal toothpaste and its extracts,there is no unified standard or specification for quality management of the effective ingredients in it,and the quality of Chinese herbal toothpaste cannot be controlled to a certain extent.In this study,the definite efficacy of mastiche extract was combined with the toothpaste system to prepare a functional toothpaste with antibacterial effect.The best technology,antibacterial parts and formula matrix were selected,and strict quality standards were established to realize the controllable and measurable quality of mastiche toothpaste.Method1.Research on the extraction process and antibacterial parts of mastiche.The extraction process of the volatile oil,water extract,and alcohol extract of mastiche was optimized,and different extracts were obtained with the best extraction process,and the antibacterial experiment in vitro was used to screen the extract of mastiche with the best antibacterial effect.2.Toothpaste matrix screening and molding process research.Use rheometer and texture analyzer to characterize the rheology and texture characteristics of toothpaste blank matrix,use MATLAB R2018b software to conduct collinearity analysis on rheology and texture characteristics,and combined with the matrix solubility,striping,foaming and other parameters,using mapminmax function to preprocess all parameters,through comprehensive analysis of the parameters,the best blank matrix is selected.Combining literature research and the results of the minimum inhibitory concentration,the amount of the extract is determined,and the wet sol paste preparation process is used to prepare the finished mastiche toothpaste.3.Research on the quality standard of mastiche toothpaste.Thin-layer chromatography(TLC)and high-performance liquid-evaporative light scattering detection(HPLC-ELSD)were used to establish the thin-layer identification and characteristic map method of mastiche toothpaste,and the pretreatment method of mastiche toothpaste was investigated through single factor experiments.And combined with GB/T8372-2017 toothpaste standard to study the quality of mastiche toothpaste.Result1.Determine the antibacterial position and the best extraction process of mastiche.The antibacterial activity of different extracts of mastiche was determined with the help of in vitro antibacterial test.The minimum inhibitory concentration of the volatile oil,water extract and alcohol extract of mastiche against Helicobacter pylori was 1 mg·m L^-1 and 0.5 mg·m L^-1,and 0.125 mg·m L^-1,respectively.And the minimum inhibitory concentrations for clinically resistant strains were 1 mg·m L^-1,0.25 mg·m L^-1,and 0.0625 mg·m L^-1,respectively.The antibacterial effect of the alcohol extract of mastiche is the most significant,so it is determined that the alcohol extract is the antibacterial part,and the best extraction process is to add 6 times the amount of 85%ethanol,reflux and extract for 30 minutes.2.Complete the screening of toothpaste matrix and determine the preparation process of mastiche toothpaste.According to the test and analysis results of physical parameters,7 parameters including yield stress,loss factor,complex viscosity recovery ratio,elastic recovery index,cohesion,wire drawing distance,and consistency are finally retained,and the No.8 blank matrix is optimized by comprehensive analysis.According to the calculation of the minimum inhibitory concentration,the addition amount of the alcohol extract of mastiche is 1%,combined with the best blank matrix formula,the finished product of mastiche toothpaste is obtained.The product has a fine paste,bright color,cool odor and moderate consistency.3.The quality standard(draft)of mastiche toothpaste is determined.With the help of thin-layer chromatography and high-performance liquid-evaporative light scattering detection technology,a thin-layer identification and characteristic map method of mastiche toothpaste was established.The obtained thin-layer chromatogram has clear spots and good resolution,and each peak of the characteristic map has good resolution and baseline.It is stable and its ingredients can be fully detected.It can effectively evaluate the quality of mastiche toothpaste.And after physical and chemical inspections,the quality standard of mastiche toothpaste was initially established.ConclusionThe research on this subject shows that the strongest antibacterial activity in mastiche is the alcohol extract,which can effectively inhibit the activity of pathogenic bacteria.On this basis,a mastiche toothpaste can be developed to prevent oral diseases.Through the quantitative characterization of the physical properties of toothpaste,the comprehensive evaluation of toothpaste performance has been achieved,and the formulation process and quality standard research have shown that the compatibility of mastiche and toothpaste matrix is stable,and the prepared mastiche toothpaste paste is delicate in body,bright in color,cool in smell and moderate in consistency.According to the established quality control method,the quality of mastiche toothpaste can be effectively controlled,which lays a solid foundation for the development of safe and effective functional toothpaste products,and is of great significance to the development of Chinese herbal toothpaste in my country.