Studay On Rapid Analysis Method Of Starch Sugar Of Preparation Process

Starch products with high added value,especially the deep processing of starch sugar and starch sugar alcohol,have a huge development prospect.It is very important to control the process and analyze the product quality.The existing analysis methods of starch and sugar can not meet the needs of practical production in terms of analysis efficiency,anti-interference and analysis cost.In this paper,a fast multi-component analysis method of starch and sugar production and HFCS products was established by combining the Angle conversion method of near infrared spectroscopy and Raman spectroscopy.The main contents are as follows:(1)Rapid analysis of fructose in high fructose corn syrup by Raman spectroscopy combined with Angle conversion.A series of fructose standard solutions with concentration gradient were prepared with commercial HFCS as mother liquid.Raman spectra were collected,and the characteristic bands of 300-1500 cm^-1were interception of Raman spectra after base line correction by fluorescence fading differential method and derivative smoothing by first-order filtering.The variances of the series of included angles between each sample and the reference sample were calculated by the subspace Angle transformation algorithm.The correlation model between the fructose content and the EE value in the sample was established,and the correlation coefficient reached 0.9991.The average relative standard deviation(RSD)of fructose in fructose syrup was1.22%,and the relative error was between 1.89%and 6.77%compared with the results determined by resorcinol-spectrophotometry.The results showed that the combination of Raman spectroscopy and Angle conversion stoichiometry had high efficiency,good accuracy and precision,and realized the quality control of HFCS.(2)Raman spectroscopic analysis of glucose isomerization.Preparation of certain content of fructose isomerization reaction liquid,as a mother liquor preparation a series of fructose concentration gradient of the standard solution,Raman spectra,after fluorescence fade split/line correction,intercepting characteristics of band,300-1500cm^-1screening model Angle conversion algorithm combining with correlation coefficient of the optimal noise intensity and mobile computing window width,glucose,fructose content in isomerization process is established rapid analysis method,correlation coefficient of 0.9903.The orthogonal test of glucose isomerization was designed.The results showed that the average absolute error was 0.51%and the average relative error was 4.49%.The optimum conditions for isomerization of glucose syrup were determined as p H:7 by range analysis.Reaction temperature:60?;Glucose mass fraction:55%;Addition of enzyme:10 mg/g glucose.Finally,the rapid analysis method was applied to the analysis of glucose isomerization.The results show that this method can achieve the direct quantification of the system spectrum without complex modeling,simple operation and low modeling cost.(3)Fast analysis of maltose content in starch liquefaction by near infrared spectroscopy combined with Angle conversion.The starch liquefaction samples were collected under different reaction time,and the maltose content of each sample was analyzed by HPLC.Near-infrared spectral data were also collected.Six samples were selected as modeling samples according to the interval of maltose content from low to high,and the rest were samples for method verification.The variance value EE of the cosine of the included Angle between the modeling sample and the reference spectral vector was calculated through the Angle conversion algorithm,and the correlation model between maltose content and EE was established.The correlation coefficient of the model was 0.9953.The EE value of the verified samples was calculated under the determined data processing parameters,and then substituted into the equation to obtain the maltose content.The relative error was less than 4.52%and the average relative standard deviation was 1.13%.Finally,the method was applied to the rapid analysis of maltose content in starch liquefaction liquid at different reaction temperatures,which provided a method reference for controlling the quality of starch liquefaction products.(4)Fast analysis of glucose content in starch liquefaction during saccharification by near infrared spectroscopy.Fixed conditions of starch liquefaction liquefied liquid saccharification experiment,the timing acquisition saccharification liquid samples,and sample collection series of near infrared spectroscopy,combined with the Angle conversion method to calculate the sample spectra with the reference spectral vector included Angle cosine series variance,EE values associated with glucose content equation,according to the model of the calculation of correlation coefficient to choose the best moving window width is 1/23,glucose content analysis model is set up by the correlation coefficient of 0.9978.The saccharification solution with different glucose content was prepared under different technological conditions,and the high performance liquid chromatography was used as the model verification method.After error analysis,the average relative error was 5.35%,and the average relative standard deviation was 3.79%.Finally,the established rapid analytical method was applied to the saccharification process at different reaction temperatures to realize the real-time determination of fructose content in the saccharification solution.