Electrospinning Preparation And Properties Of Zein-based Porous Fiber Membrane Compounded Nisin

Corn is one of the world's three major food crops,and is an important feed raw material,corn has a high yield per unit area and a great potential to increase production,has a pivotal position in agriculture,China's annual output of corn will reach 260 million tons in 2020.In this paper,through the use of zein as the main raw material,adopt the method of electrostatic spinning get zein nano fiber membrane,the streptococcus and nanofiber membrane composite together,through the nano fiber membrane soaking in streptococcus element in the solution and the zein mixed with streptococcus element solution spinning of the reentry after get nano fiber membrane,the antiseptic antibacterial performance were studied.The properties of the two kinds of nanofiber membranes were explored,and finally the nanofiber membranes were combined with streptococcus lactate,and the antibacterial properties of the nanofiber membranes with streptococcus lactate composite using the two methods were compared.Specific research contents are as follows:(1)Preparation of fiber films by uniaxial and coaxial electrospinning of Zein and polyamideacid and their properties:The optimum spinning conditions were determined by exploring the viscosity and surface tension of the solution of Zein and polyamide acid.The composite nanofiber membrane was obtained by Fourier transform infrared spectroscopy,and the decomposition temperature of the nanofiber membrane was determined by thermogravimetric analysis.The decomposition temperature of zein was about 280?,and the decomposition temperature of polyimide was about 505?.Calcination in muffle furnace,calcination time of 2h is the best effect,find the best calcination scheme.After being calcined at high temperature and for 2h,the surface of the fibers was observed to have pores by means of field emission scanning electron microscopy.The specific surface area of the nanofiber films obtained under uniaxial and coaxial conditions was determined.The specific surface area of the porous polyimide nanofiber films under uniaxial and coaxial conditions was 304.6313m~2/g and 37.3504 m~2/g,respectively.The nanofiber membrane was immersed in nisin solutions of different concentrations for 8 hours,and the nisin-loaded porous nanofiber membrane was used for antibacterial.(2)By mixing zein with polyethylene oxide and adding different proportion of streptococcus lactide in uniaxial electrospinning,the nanofiber films loaded with streptococcus lactide were obtained.The influence of spinning process on the diameter of nanofibers was investigated by setting different spinning voltage,receiving distance and feeding rate.The effects of adding different amounts of streptococcus lactate on the viscosity and surface tension of the solution were observed.The conductivity of the solution increased with the increase of the lactococcin ratio.Streptococcus lactate has little effect on the thermal decomposition curve of the nanofiber membrane,and the thermal properties of the nanofiber membrane are relatively stable.Fourier transform infrared spectroscopy confirmed that streptococcus lactate was successfully loaded in the nanofiber membrane.The tensile stress and elongation at break of the nanofiber films obtained by electrospinning both increased firstly and then decreased with the increase of streptococcus lactate content.Antibacterial experiments show that adding 1%and 5%lactic streptococcus element of nanometer fiber membrane on the staphylococcus aureus was not remarkable bacteriostasis,add a concentration of 10%zein film on lactic streptococcus a reputation of staphylococcus aureus have strong antimicrobial activity,and adding amount of 15%lactic streptococcus element of zein fibre membrane within 24h can inhibit the growth of staphylococcus aureus,completely failed to detect residual total number of bacteria colonies.This experiment provides a new idea for the application of zein,and comprehensively utilizes the by-products of corn starch production.At the same time,the loading of streptococcus lactate also functionalized the nanofiber membrane,highlighting the excellent performance of the nanofiber membrane,so that the nanofiber membrane we obtained can be studied for its specific applications,and improve the application range of zein and nanofiber membrane.The electrospinning of zein and the anti-corrosion and antimicrobial properties of food packaging materials provide abundant experimental data,which will lay a favorable technical support for the food packaging and storage process in the future,and will also promote the electrospinning load of functional active ingredients and other related research work.