Study On DMP,DBP And DEHP Migration In Curdlan Membrane And Its Safety Evaluation

Plastic packaging materials not only waste oil resources,but also pollute the environment.Therefore,the development of new green bio-based packaging materials has become an urgent requirement.Curdlan are the extracellular polysaccharides produced by fermentation of Alcaligenes faecalis.When the temperature reaches 60?,it forms a kind of low intensity gel which possesses the thermal reversible.When the temperature reaches 80?,it forms a kind of high-strength gel which has a strong thermal irreversible.Curdlan are the ideal source of natural polymer film materials and bio-based packaging materials.But the mechanical properties of the membrane made of Curdlan is poor.So,we need to add plasticizer to Curdlan film.Phthalates plasticizer have been widely used in food packaging.But as time goes on,due to the increase in temperature or mechanical stress,the plasticizer will be transferred from the plastic packaging material to the food or beverage.Phthalates plasticizer are considered to be endocrine disruptors(EDs),which may cause cancer in the reproductive system of human.At present,there are few studies on the migration of plasticizers in bio-based packaging materials.Therefore,it is very meaningful to develop a safe biodegradable Curdlan film and study the migration rule of phthalate plasticizers.This subject is a sub-project of the National Key Program(2018YFD0400703)"Research and Development of New Food Packaging Materials and Intelligent Packaging Key Equipment".The main research content of this paper is the study of the migration rule and safety evaluation of plasticizers in new bio-based food packaging products.DMP,DBP and DEHP were added to the Curdlan to form a film and study the migration rule of the three plasticizers.Then,the safety evaluation and performance measurement of the film were conducted.The main results were as follows:1.A new type of Curdlan film with DMP,DBP and DEHP was developed.According to GB31604.30-2016 "Food Contact Materials and Products Determination of Phthalates and Method of Migration",the amount of migration of three phthalate plasticizers in water,4% acetic acid,50% ethanol and n-hexane were measured by external standard method.It was found that the order of the amount of migration in the four food simulants is:n-hexane>50%ethanol>4%acetic acid>water.When the amount of plasticizer added is 3%,the migration of DMP in Curdlan basilemma in water,4%acetic acid,50% ethanol and n-hexane are 30.96,44.38,56.21 and 81.38mg/kg,respectively.The migration of 3 plasticizers showed an increasing trend in three days.Over time,the amount of migration gradually increases.The migration balance was reached on the third day,and the migration amount reached the maximum value and remained stable.As the temperature increases,the migration of plasticizer increases.The higher the concentration,the greater the migration.2.The Weibull migration model of DMP,DBP and DEHP in Curdlan membrane in n-hexane food simulant were established below:(?)The C_m,? and ? are three fitting parameters,which are greater than zero.The C_m is a function of the plasticizer concentration and ? is a function of temperature.Beta,a certain value,has no direct connection with temperature and its change has no effect on the precision of the model.The migration of the three plasticizers conform to the following formula.Among them,the relationship between the migration parameter ?₀ and T of DMP in n-hexane is ?₀=exp(0.1294T-70.0346),and the correlation coefficient R² is 0.9931.Beta is the average value 1.30.The relationship between C_m and concentration x is C_m=1545.96x+2.16 at 4? and the R² is 0.9735.The relationship between C_m and concentration x is C_m=2106.58x+3.46 and the R² is 0.9749 at 25?.The relationship between C_m and concentration x is C_m=2526.32x+9.02 at 40? and the R² is 0.9714.The relationship between DBP migration parameter ?₀ and T in n-hexane is ?₀=exp(0.1248T-76.7756)and the R² is 0.9985.Beta is the mean value 1.18.The relationship between C_m and concentration x is C_m=1251.82x-0.39 at 4?.The correlation coefficient R² is 0.9893.The relationship between C_m and concentration x is C_m=2037.36x+0.24 at 25 ?.The correlation coefficient R² is 0.9852.The relationship between C_m and concentration x is C_m=2459.7x+8.23 at 40?.The correlation coefficient R² is 0.9604.The relationship between DEHP migration parameter ?₀ and T in n-hexane is ?₀=exp(0.1686T-100.6162).The correlation coefficient R² is 0.9986.Beta is the mean value 1.29 and C_m and concentration x the relationship is C_m=679.29x+3.89 at 4?.The correlation coefficient R² is 0.9932.The relationship between C_m and concentration x is C_m=697.91x+6.57 at 25 ?.The correlation coefficient R² is 0.9984.The relationship between C_m and concentration x is C_m=703.44x+11.45 at 40 ? and the R² is 0.9546.(?)3.Packaging real sauce-like foods with Curdlan films of different plasticizer concentrations.The migration of 3 plasticizers in three types of real sauce-like foods,"Heinz tomato sauce"(water-based sauce-like food),"Junlebao original old yoghurt"(acid sauce-like food)and "Lao Ganma oil-made chili sauce"(oily sauce-like food)were measured.At the same time,the activity of the cell dealed with Curdlan suspensions which contain different plasticizer concentrations for 24,36 and 48h were measured.Only when the plasticizer concentration was 0.1%,the cell survival rate was over 80%,which indicated the Curdlan films contained 0.1% plasticizer concentration have no cytotoxicity and meet the safety requirements.The properties of the Curdlan membrane with 0.1% plasticizer content and the pure Curdlan bio-based membrane without plasticizers were measured.Only the water absorption of the biofilms was not significantly different,the film mechanical properties,water vapor transmittance and transparency were significantly different.