Study On Biological Enzymatic Hydrolysis Of Cynanchum Auriculatum Royle Ex Wight Beverage And Its Act Function

Cywawchm auriculatum Royle ex Wight is rich in crude protein,free sugar,starch,free amino acids,inorganic salts,minerals and other nutrients,as well as bioactive components such as Cynanchol,C21 steroidal aglycone,phenylketones and polysaccharides.It has the functions of inhibiting tumor growth,anti oxidant and anti free radical,improving immunity and reducing blood lipid.In this paper,the biological enzymolysis technology was used to break the wall of Cywawchm auriculatum Royle ex Wight,and a kind of functional beverage with high added value was obtained,which provides theoretical data for the development of Cywawchm auriculatum Royle ex Wight in the food production industry.In this paper,the processing technology of biological enzymolysis of Cywawchm auriculatum Royle ex Wight was optimized,the antioxidant activity in vitro and the ability of lowering blood lipid in vitro were evaluated,and the Cywawchm auriculatum Royle ex Wight beverage with active function was developed.The specific research contents and experimental results are as follows:1.Through the screening and compounding of the compound wall breaking enzyme of Cywawchm auriculatum Royle ex Wight,and the mixture analysis of the compound enzyme ratio,the best compounding ratio is:cellulase:pectinase:xylanase=11:5:4.Under these conditions,the content of soluble solids in the hydrolysate was 3.95%±0.05%,and the total sugar was 10.85± 0.96ug/ml.Through the analysis of the conditions of enzymatic hydrolysis of Cyuawchm auriculatum Royle ex Wight by the optimization of response surface,the optimal enzymatic hydrolysis process of Cywau,chm auriculatum Royle ex Wight was obtained as follows:enzymatic hydrolysis time 4h,enzymatic hydrolysis temperature 75?,enzymatic hydrolysis pH5.5.Under the optimized conditions,the content of soluble solid and total sugar in the hydrolysate of Cywawchm auriculatum Royle ex Wight was 4.69%±0.02%and 12.10 ± 0.15ug/ml respectively.At the same time,the content of flavone and total phenol in the liquid of Cywawchm auriculatum Royle ex Wight was 57.5%and 68.6%higher than that of the control group.2.ABTS free radical scavenging ability,DPPH free radical scavenging ability,reducing power and ORAC were used to analyze and study the in vitro antioxidant capacity of Cywawchm auriculatum Royle ex Wight hydrolysate.The IC50 of DPPH·free radical and ABTS+free radical were 0.273mg/ml and 0.053mg/ml respectively;When the concentration reached 1mg/ml,the reducing power of the hydrolysate reached 2.576;The ORAC value of the hydrolysate was 791.46 ±9.81umol Trolox/g,indicating that the hydrolysate had strong antioxidant capacity.Through the study on the ability of absorbing bile acid salt and cholesterol in vitro,we found that the binding amounts of the enzyme solution to sodium cholate,3.sodium taurocholate and sodium glycine were 0.752±0.006umol,0.580±0.010umol and 0.573±0.007umol,respectively;The adsorption capacity of Cywawchm auriculatum Royle ex Wight hydrolysate to cholesterol was 5.28 ±0.04mg/g in the stomach digestion environment with pH=2,and 8.94±0.06mg/g in the intestine digestion environment with pH=7.It has good absorption capacity of cholic acid and cholesterol reduction capacity.4.Through the screening and proportioning of the composite stabilizers added in the development of Cywawchm auriculatum Royle ex Wight functional drink,the proportioning of the composite stabilizers is obtained as follows:guar gum:sodium alginate=1:1,the composite stabilizers prepared according to the formula,the viscosity of the drink is 8.97cp,the suspension stability is 42.40%,and the stabilization effect is the best.With the enzyme hydrolysate of Cywawchm auriculatum Royle ex Wight as the raw material,the formula of Cywawchm auriculatum Royle ex Wight functional drink was optimized by response surface method.The best formula was as follows:the content of Cywawchm auriculatum Royle ex Wight original liquid was 30%,the content of white sugar was 4g/100mL,the content of composite stabilizer was 0.10%.The prepared Cywawchm auriculatum Royle ex Wight functional drink had unique aroma,mellow taste,sweet taste and even color.The pH value,soluble solid content,total bacterial count and sensory score were used as indicators to explore the trend of the change of Cywawchm auriculatum Royle ex Wight functional drink at 40?,27? and 37?,respectively.The stability of Cywawchm auriculatum Royle ex Wight functional drink at 4? was the best,and the microbial index was lower than the national standard.The shelf life of the drink at 20? was estimated to be about 166 days by accelerated shelf life prediction experiment.Finally through the determination of physical and chemical indicators and microbiological indicators,the product quality of Cywawchm auriculatum Royle ex Wight functional drink was tested,which met the national standards.