Research Of Phyllostachys Heterocycla Dyed By Penicillium Griseofulvum And Trichoderma Viride

The biological discoloration of bamboo is common,and it is usually considered as a defect.In recent years,more and more attention has been paid to the study of wood biological dyeing,but there is still a blank in the functional development of bamboo.The traditional bamboo dyeing process mostly uses wood dyeing method,which easily leads to high energy consumption and high pollution.Therefore,it is of great significance and value to explore a kind of green and natural bamboo biological dyeing technology by using the characteristics of biological discoloration of bamboo.In this study,based on the biological dyeing technology of wood and the theory of the formation of bacteria lines,Penicillium griseofulvum was selected as the"dye",Trichoderma viride as the"catalyst",and Phyllostachys heterocycla as the dyeing object.Firstly,the biological characteristics and growth rate influencing factors of Penicillium griseofulum were studied,and the most suitable medium conditions for growth and secretion of Penicillium griseofulum were obtained.The surface properties,physical and mechanical properties,infection path and chemical changes of Phyllostachys pubescens dyed by Penicillium griseofulum alone were studied.The antagonistic effect between Penicillium griseofulum and Trichoderma viride was verified,and the synergistic dyeing of Penicillium griseofulum/Trichoderma viride was prepared Finally.The production process of dyeing bamboo was optimized,and the optimized process was compared with the ordinary dyeing process.The main conclusions are as follows:(1)The results showed that the optimal culture conditions of Penicillium griseofulum were glucose 40 g/L,ammonium sulfate 6 g/L,K₂HPO₄2 g/L,Fe SO₄0.03 g/L,Mg SO₄2.0 g/L and agar 15 g/L.The growth rate and pigment secretion of Penicillium griseofulum were enhanced by PDA culture medium than the optimal culture medium,so PDA culture medium was selected to culture Penicillium griseofulum.(2)The surface color of Phyllostachys heterocycla infected by Penicillium griseofulum gradually changed to dark red,and the color difference before and after dyeing was strong,forming a good decorative effect.The hydrophobicity of dyed Phyllostachys heterocycla was improved,the roughness had no change,the mass loss rate increased slightly,and the water absorption and compression strength decreased slightly.Penicillium griseofulum entered into the interior of Phyllostachys heterocycla through the catheter,and the colony secreted exudate on the surface of Phyllostachys heterocycla,and then recovered The chemical components of dyed Phyllostachys heterocycla were degraded.(3)The antagonistic effect between Penicillium griseofulum and Trichoderma viride was found by plate confrontation experiment and microscopic examination,so the phenomenon of a large number of pigments produced at the intersection of the two bacteria was in line with the theory of the formation of bacteria lines.The synergistic dyeing of Phyllostachys heterocycla was obtained by using the pre-infection of Trichoderma viride to stimulate Penicillium griseofulum to secrete a large number of pigments on the surface of Phyllostachys heterocycla;the comparative test showed that the synergistic dyeing of Phyllostachys heterocycla could be dyed by Penicillium griseofulum.On the other hand,the similar dyeing effect can be obtained in a shorter time,and the degradation and mechanical properties of Phyllostachys heterocycla can be reduced.(4)The optimal co-dyeing process of Phyllostachys heterocycla was as follows:Trichoderma viride was cultured for 3 days,and then pre-infected for 6 days.The visible hyphae on the surface of Phyllostachys heterocycla were removed with sterile brush,and then cultured for3 days with Penicillium griseofulum,and then infected for 9 days.Compared with acid scarlet dyeing,the surface color difference of Phyllostachys heterocycla dyed by the optimized process is stronger,the washing fastness is higher,and it has the advantages of green,natural and efficient.