| Aging is the aging process of various organs of the body as living organisms age,and is interacted with genetic inheritance and acquired environmental influences.Aging is not only reflected in the appearance characteristics,but also in the ability to exercise,anti-environmental stress,cellular metabolism level,reproductive ability and so on.Aging will be accompanied by the occurrence of various diseases,and people pay more and more attention to the research of anti-aging.A large number of experiments have shown that anti-aging natural products mostly have antioxidant,anti-tumor and other effects.Most of the natural products are plants,animals or microorganisms with medicinal value and related secondary metabolites.Natural medicines have complex ingredients,few side effects,and are readily available on the market.The use of fruit fermentation liquid(FFL)is based on guava,dragon fruit and emblica as raw materials,obtained after fermentation by lactic acid bacteria,containing polysaccharides,vitamins and a variety of trace elements and other nutrients.In order to study the anti-aging effect of the product in depth,we used Caenorhabditis elegans as a research object to explore its anti-aging and other related effects.Caenorhabditis elegans is a commonly used model organism for studying aging,which is characterized by a short life cycle,simple and easy to cultivate,and sensitivity to environmental changes.Firstly,it was determined that the fruit fermentation liquid did not restrict the growth of E.coli,and did not lead to the extension of the life of nematodes due to caloric restriction;secondly,the changes in the life cycle,lipofusin accumulation,egg production,and mobility of wild-type Caenorhabditis elegans after eating fruit fermentation liquid were evaluated.The results showed that compared with the blank group,the lifespan of nematodes acting on fruit fermentation liquid was about20% longer;and the accumulation of lipofuscin in 10-day-old nematodes was reduced by about 76% year-on-year.There was no negative effect on reproduction,and the number of eggs laid was very similar.The peak spawning period of the fruit fermentation broth(FFL)group was one day later than that of the control group;the spawning period of the 50%FFL group and the 25%FFL group did not change.The exercise ability of the nematodes was tested on the 4th,6th,8th,10 th and 12 th days.The exercise ability of the FFL group was significantly enhanced compared with the control group,and the cosine swing frequency of the nematodes was also higher than that of the control groupFinally,in order to further study which way nematodes may delay aging,we tested oxidative stress and heat stress experiments,and detected the ROS level in nematodes by DCFH-DA probe to explore the anti-aging mechanism of fruit fermentation broth.The results showed that,under oxidative stress and high temperature environment,the survival of the FFL group was significantly better than that of the control group,which improved the survival rate of nematodes under adversity;the level of ROS decreased by nearly 60%.This shows that the fruit fermented liquid has a good anti-stress protective effect,and delays aging by reducing the accumulation of its free radicals.On this basis,the fat accumulation of nematodes was detected,and it was found that the fruit fermentation broth accelerated the metabolism of nematodes,reduced fat accumulation,and delayed the aging of nematodes from the metabolic pathway.In summary,in this paper,nematodes were cultured with a variety of fruit fermentation broths,and it was observed that the nematodes had enhanced stress resistance to adversity,decreased ROS accumulation levels,and decreased fat accumulation.Lay the foundation for research on the impact on human lifespan. |
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