The Separation And Extraction Of Red Pigment Lonicera Edulis And The Study On The Technology Of Its Sustained-release Microencapsulation

Lonicera edulis belongs to the Caprifoliaceae and Lonicera L. in plant taxonomy. Proved by the clinical trials, it has certain curative effect on the cardiovascular and cerebrovascular diseases. And also it can resist some viruses and tumors, and improve the antidotal function of liver.Red pigment Lonicera edulis is extracted from the natural Lonicera edulis and is widely applied in food, beverage and cosmetics, with higher edible and medicinal value. Red pigment Lonicera edulis has less stability and greatly influenced by the external factors such as light, temperature, pH, etc. we can improve its stabilization and applied scope by technology called the releasing polythiourea microcapsule.In the trial, through several single factor experiments, study the affect of the extractive solvent, extraction concentration, solid to liquid ratio, extraction temperature and extraction time on the extraction of red pigment Lonicera edulis were studied, and then the conditions were optimized by orthogonal experiment and ultimately the optimum conditions for the extraction of red pigment Lonicera edulis were determined:the extraction agent was acidification ethanol, the extraction temperature was 50℃, the extraction time was 1.5h, the concentration of acidification ethanol was 60% and the solid to liquid ratio was 1:15. The crude extraction solution of Lonicera edulis was refined through X-5 macroporous resin, and higher purity red pigment Lonicera edulis was obtained.During preparation of the microcapsule using red pigment Lonicera edulis, we can use the Piercing-solidifying Meth. the best ratio of the mixed wall material is gelatin and the sodium alginate was determine which was 1:2, the optimal concentration of wall material was 3%, the optimal ratio of core material and wall material was 1:3, the optimal concentration of CaCl2 was 2.5%, the optimal solidify time was 50min. On the basis of the above, response surface analysis was done and the optimum conditions for microencapsulation were determined:the ratio of gelatin and sodium alginate was 1:2, the concentration of wall material was 3.22%, the ratio of core material addition and wall material addition was 1:3.21 and the concentration of CaCl2 was 2.40%, the embedding rate could reach 88.39%.Subsequent tests proved that the stability of the microen-capsulated red pigment Lonicera edulis had improved compared to these did not microen-capsulated red pigment Lonicera edulis in the same conditions, such as light, temperature and pH. The release process of microcapsules was determined by simulated gastric and intestinal environment, and fitted with zero-order kinetics, first-order kinetics and Higuchi equation, the results showed that the order of the release kinetics fitting results of red pigment Lonicera edulis microcapsules in the gastric and intestinal environment both were Higuchi model equation, first-order kinetics, zero-order kinetics. The release process of red pigment Lonicera edulis microcapsules in gastric and intestinal environment was matrix diffusion mechanism which followed Higuchi model equation.