A Novel Hollow Tube White-rot Fungal Reactor: Its Developement Based On Vascular Carrier And Continuous Operation

Based on vascular carrier and Zheng-Long reactor,our research group has successfully achieved the long-term continuous treatment of landfill leachate and coking wastewater using white-rot fungal treatment system.However,Phanerochaete chrysosporium aerobic filamentous biofilms,covered on the surface of vascular carrier,were torn open and exfoliated due to the air-bubble and the flow of wastewater.In order to solve this problem,the vascular carrier has been improved to form the P.chrysosporium aerobic filamentous biofilm,which used to continuously treat landfill leachate in a novel hollow tube white-rot fungal reactor.Furthermore,the stability of P.chrysosporium aerobic filamentous biofilm in/on the improved vascular carrier was investigated.The experimental results showed that:1)The long-term continuous operation of the hollow tube white-rot fungal reactor was achieved.Under non-sterile conditions,the continuous 401 day（18batch）growth of P chrysosporium aerobic filament biofilm in hollow tube white-rot fungal reactor was successfully achieved.The lignin peroxidase（Li P）can be continuously synthesized and its activity is maintained at 33 U/L to 199 U/L.During18 successive batches,P.chrysosporium aerobic mycelium biofilm achieved 56～74%COD removal rate(removed from 6 545 mg·L^-1to 1 701～2 888 mg·L^-1),83～97%ammonium nitrogen removal rate(removed from 2 969 mg·L^-1to 80～511 mg·L^-1)and 009～2.35 g·batch^-1of discharged biosludge.2)The stability of P.chrysosporium aerobic filamentous biofilm on the improved vascular carriers was enhanced.In landfill leachate,some outer layers of P.chrysosporium aerobic filamentous biofilms were torn open in 60-day operation of hollow tube white-rot fungal reactor and gradually exfoliated,but most of outer layers of P.chrysosporium aerobic filamentous biofilms were torn open and were not exfoliated during 18 successive batches,which was attributable to（1）the improved vascular carriers,designed to form aerobic filamentous fungal biofilm in/on its surface and to achieve the continuous supply of nutrient and oxygen for its inner layer to keep its continuous growth;（2）the hollow tube white-rot fungal reactor,designed to control the flow direction of air-bubble and wastewater,which avoided them direct effects to P.chrysosporium aerobic filamentous biofilm,decreased some damages for biofilm and then achieved the stability of P.chrysosporium aerobic filamentous biofilm on carriers.All these results suggested that the improved vascular carriers and the hollow tube white-rot fungal reactor can be used to achieve continuous growth of P.chrysosporium aerobic filamentous biofilm and to improve its stability in/on carriers,which is foundational for treating the wastewaters using white-rot fungi.