Development Of Biogas Residue Based Bio-organic Fertilizer With Compound Bacterial Strains

With the national sustainable development policy and the wide popularization of people’s awareness of ecological and environmental protection,the agricultural production in our contryis becoming more and more aware of fertilization,soil quality and sustainable development,which makes the development and research of new fertilizer gradually become a hot spot.The bio-organic fertilizer and microbial agent produced according to its properties are widely used in agricultural production.In this study,beneficial bacteria were recurited in cucumber splited rooting experiment under infection with pathogen,a rhizosphere growth promoting strain FE36 with high yield of IAA production and an antagonistic strain M8 were screened.The new type of bio-organic fertilizer was developed by combining these two strains with the growth promoting Bacillus ameyloliquefaciens strain SQR9.Fusarium wilt is a kind of highly destructive fungal crop disease caused by Fusarium oxysporum.Combined with pot experiment,the effect of biogas residue organic fertilizer on cucumber growth was studied.The following results were obtained:1.In this study,based on the topic search of "Fusarium wilt",4873 related literatures from 1985 to 2019 were searched in the core set of ISI Web of science database.CiteSpace visualization software was used for citation analysis.Through cooperation,co-occurrence,co-citation and other network analysis,the research hotspot and cutting-edge trend of Fusarium wilt were studied.According to the intermediary central statistical analysis,The United States,China and India rank in the top three in this field,while the analysis of intermediary centrality index shows that the Netherlands,The United States and India rank in the top three in the importance of literature.China ranks in the top 10 and shows a positive upward trend,with Nanjing Agricultural University posting the most.The key words of citation analysis are "induction","genetic diversity" and "pathogenicity" which are important hot spots in recent ten years.The cluster analysis of literature cited shows that the research of Bacillus amylolyticus,systemic resistance and non pathogenic Fusarium oxysporum is the research frontier in this field.With the deepening of the research level of Fusarium wilt,the future research will focus on microbial control,disease resistance breeding,root pathogen beneficial bacteria interaction mechanism,etc,in addition,the application of multicomponent technology will be conducive to the analysis of Fusarium wilt pathogenesis,and provide theoretical and technical support for the basic research and control application of Fusarium wilt.2.A large number of Bacillus and Pseudomonas were screened out from cucumber roots through the recruitment under infection of pathogen.The IAA producers were obtained to verify the plant growth promotion efficiency and antagonistic ability of the strains against Fusarium oxysporum was also measured.The results were as follows:the highest IAA producing strain FE36 was picked out by salkowski color reaction test;the antagonistic strain M8 with the best antagonistic effect on Fusarium oxysporum was picked out by plate confrontation method;the strains FE36 and M8 were identified to be Bacillus gorilla and Pseudomonas aeruginosa,respectively.3.The acidolysis amino acids were added to the biogas residue fertilizer,and the screened antagonistic bacterial strain M8 and growth promoting bacterial strain FE36 together with Bacillus ameyloliquefaciens SQR9 were added to biogas residue,and then biogas based complex microorganisms bio-organic was made for further evaluation.The nutrient content was 2.8%nitrogen,2.6%phosphorus,0.6%potassium and 63.2%organic matter.The number of three strains in the compound biogas residue bio organic fertilizer was higher than 2×10~7 CFU/g,and the moisture content was lower than 30%,which reached the national standard.4.In the pot experiment,the growth promoting effects on cucumber was carried out with the compound microbial biogas residue fertilizer developed with the functional bacteria M8,FE36 and SQR9.The plant aboveground height of the bio-orgnic fertilizer treatment was 84%higher than that of the biogas residue fertilizer treatment;the stem diameter phase was 14%higher;the fresh weight of the aboveground and the belowground was 74%and 34%higher;the dry weight of the upper part and the underground part increased by 21%and 25%respectively,the chlorophyll content increased by 9%,and the effect of promoting growth was obvious.The pot experiment indicated significant promoting growth and disease inhibiting effects of the biogas residue based complex microorganisms bioorganic fertilizer.