Development Of Strain Loading Device Based On Neural Tissue Engineering

The incidence rate of central nervous system injury has been increasing in recent years.Transplantation of neural stem cells can play an important role in the treatment and rehabilitation of injured central nerve,but it is difficult to repair injured peripheral and spinal cord.Most studies on axon growth believed that the early stage is the most important stage of nerve tissue growth.But recent studies have proved that nerve tissue continues to grow rapidly in the process of animal development.Therefore,the culture of stem cell and neural tissue engineering are very important for repairing injured peripheral and spinal cord.In this paper,the development of neural tissue engineering and its bioreactor were reviewed,and a strain loading device for neural tissue engineering was developed.The device compriseed a worktable,a power mechanism,a mechanical loading mechanism and a cell culture mechanism.When the device works,the loading mechanism wass pushed by the power mechanism,and the unidirectional strain load wass applied to the cell culture mechanism to stimulate the axon growth of nerve cells.In this paper,the mechanical analysis and optimization of silicone rubber tube of neural tissue engineering loading model and scaffold of neural tissue engineering culture were carried out.The study found that within 50% of the strain condition,the strain in the middle region of the scaffold material was the closest to the actual loading strain.The error range was controlled within ±3% ～±8%,and the uniform strain region of the culture environment was controlled above 74.8%.It met the requirements of cell culture.And the study analyzed the thermal conductivity of tissue engineering temperature field model of strain loading device,to concluded that the temperature of the whole device can be maintained to 36.54 ± 0.47 ℃ and the temperature of the culture tube can be maintained to 36.18 ± 0.38 ℃ when the heating block is heated to37 ℃ at 25 ℃.It met the requirements of cell growth.And computational fluid dynamics(CFD)simulations were carried out at different flow rates in the bioreactor,to concluded that when the inlet velocity is 0.5m/s,vortex can be formed at the end of the device near the outlet to improve the material exchange efficiency,and put forward the design position of gas inlet and outlet and the placement position of cell culture tube.In the final part of this paper,the study analyzed the advantages,technical innovation and disadvantages of the strain loading device,to lays a foundation for further research in the field of neural tissue engineering.