Research And Development Of Dry Fluorescence Immunoassay Device

As a quantitative detection method in the field of In Vitro Diagnosis,fluorescence immunochromatography is of great clinical significance for rapid identification of bacterial and viral infections,postoperative monitoring,risk assessment of cardiovascular diseases,and animal immunity.At present,most of the Third-Class hospitals in China use large-scale imported automation equipment,and its high price is not conducive to the introduction of community hospitals and inspection institutions,while the inspection equipment developed by domestic companies is still faced with problems such as poor analytical performance and dependence on imports for partial reagent.To solve these problems,this paper proposed a low-cost,miniaturized and portable threechannel dry fluorescent immunoassay analyzer based on embedded system,which is suitable for all kinds of medical institutions on the basis of ensuring the high efficiency and accuracy of the detection results.The specific research contents include:(1)According to the actual detection requirements,this paper analyzes and proposes a simple confocal optical system scheme,using ZEMAX to simulate and optimize the excitation and acquisition light paths,and select the light source,photodetector and optical device.After completing the selection and testing,the design of the entire optical structure is realized,laying the foundation for the accurate acquisition of fluorescence signals.(2)Propose a scheme of miniaturized mechanical system including transmission structure and optical path structure.Testing the materials used in the mechanical structure of optical path minimizes external noise interference and improves the performance of the optical path system.Then,according to the formulation of mechanical scheme,a set of hardware control circuits are designed,which mainly include the main control circuit,LED drive circuit,motion control circuit,signal acquisition and processing circuit,etc.(3)Because of the high noise of the initial fluorescence signal,a set of algorithms based on sliding average filtering and least square curve fitting is proposed to process the collected initial electrical signal,and the final detection results are calculated by the area algorithm to ensure the authenticity of the detection data and improve the sensitivity of the detection.(4)On the basis of the simplification of the detection process and the convenience of manmachine operation,this paper designs two sets of PC interaction schemes,one is a device-side test interface based on a touch screen,the other is the PC-side test interface based on C SHARP,which can meet the needs of different occasions.(5)After each module is designed,assembled and debugged,the performance of the whole machine is tested and analyzed using standard reagent strips and reference reagents,including accuracy test,linearity test and repeatability test.The three-channel dry fluorescence immunoassay device designed in this paper has the advantages of convenient operation and low cost,and the test results show that the test error of the system can be controlled within ±1%,and the linear fitting degree of the standard reagent strip can reach 0.9891,and the CV value can be controlled within 1%.Therefore,the device has high accuracy,wide linear range and good repeatability,which meets the national standard requirements of medical equipment.