Characteristic Components Of Propolis In Northeast China

Propolis is a natural active substance,is the bee plant bud and stem collected from resin,mixed with bees palate gland secretions,pollen and beeswax and other by chewing and processed into a with aromatic smell of solids.Propolis has antibacterial anti-inflammatory,antioxidant,anti-tumor,anti diabetic activity,which is close related to the chemical composition of propolis.The chemical composition of propolis is complex,and the different plant resins collected by honey bees in different climatic conditions are different.Therefore,the chemical composition of propolis from different areas is different,which leads to the different biological activities of propolis.China is one of the most important propolis producing areas in the world.The chemical composition of propolis has been widely studied.Because of the large number of Chinese production,the geographical span of large,so there is a difference in chemical composition.The current research is widely distributed in the temperate regions of the poplar type propolis,lack of system researching of other regions.therefore,In this paper,the detection and analysis of propolis samples from different regions of the whole country found that the classification of Chinese propolis was in accordance with the classification of Chinese vegetation regionalization,the characteristics of propolis of the th northeastern provinces was identified by the Chinese vegetation.By chemical method and H(?)composition of shandong propolis and Jilin propolis were compared,and the differences were found out.identify the characteristic component of dongbei propolis was identified by Using mass spectrometry and nuclear magnetic resonance spectroscopy.A HPLC method was builted for the detecting the propolis of Northeast China,which was based on characteristic components.Furthermore,The effect of on the anti ischemia reperfusion injury of P-coumaric acid benzyl ester was studied by means of N2a cells,to understand the role of P-coumaric acid benzyl esterl in the protection of the nerve.1、One hundred and Thirty-four propolis samples collected randomly from 24 provinces of China were extracted by methanol assisted with ultrasound.Through the analysis of HPLC-DAD and standard products,the characteristic component of propolis in Northeast China was found.Established a rapid classification method of propolis in China,and divided the Chinese propolis into four categories combined with vegetation regionalization of China,it also found that the characteristics of the Northeast propolisSubstance A.2、Propolis collected from jilin and shandong provience were qualitative analysised by HPLC-DAD and compared with standard products,In the case of the same quality,there were five different chemical composition found on 320nm between shandong and jilin propolis:Coffee acid,P-coumaric acid,Caffeic acid phenethyl ester,Substance A and coffee acid ester.Coffee acid and P-coumaric acid were both in shandong and jilin propolis,but had different shape in 320nm.Caffeic acid phenethyl ester and coffee acid ester are the unique characteristic components of Shandong propolis.Substance A is the unique characteristic component of propolis in Jilin propolies.3、By using thin-layer chromatography and medium pressure chromatography,preparative liquid chromatography and high performance liquid chromatography technology,Prepared more than 99%purity of northeast propolis characteristic components A.Through the detection and analysis of Chinese Medical Science Research Institute of the drug by mass spectrometry and NMR,it was P-coumaric acid benzyl ester,the characteristic components of propolis.4、Sample pretreatment method of P-coumaric acid benzyl ester and HPLC conditions were worked out.A HPLC method was builted for the detecting the propolis of Northeast China,which was based on characteristic components,through the experimental method to verify that this method meets the requirements.By using this method in the testing the content of P-coumaric acid benzyl ester of northeast propolis,the determination results was consistented with conclusion.It was basis for the classification of propolis.5、Through the simulation of cerebral is chemia reperfusion injury of N2a cells with the model of OGD4h and ogd-r 24h,the cell survival rate and cell morphology as indexes,P-coumaric acid benzyl ester was comfirmed have the good effect on the ischemia reperfusion injury of N2a cells in vitro with compared with the positive control of Ligustrazine,It provided the basis for the discovery of the protective activity of propolis in Northeast China.