| In order to explore the effects of supplementary light and light quality on ginseng growth and physiological and biochemical indicators and saponin accumulation,the three-year-old ginseng was exposed to two hours before the sun rises after the leaf spreading period and before the leaves wither and two hours after sunset,respectively The LED plant lamp was used to irradiate white light,blue light,yellow light,red light and red-blue mixed light treatment,and compared with non-filled light,the photo synthetic characteristics,antioxidant enzymes,main organic matter and growth indexes of ginseng with different light quality filled light were analyzed.1.The effects of saponin accumulation and gene expression of key enzymes that control saponins mainly draw the following conclusions:(1)Compared with the control,the five treatments in this experiment increased the net photosynthetic rate of ginseng to different degrees,the leaf stomatal conductance,transpiration rate and intercellular CO2 concentration,of which the red and blue mixed light effect was the most significant,and the net photosynthetic rate The diurnal variation of the double peak curve appears,respectively at 9:00-11:00 am and 13:00-15.00 pm;the red light treatment improves the blade light energy utilization rate,the actual electron transfer efficiency,and the red and blue mixed light Reduced heat consumption during the electron transfer process.(2)The supplementary light treatment increases the activities of ginseng catalase,peroxidase and superoxide dismutase to different degrees,of which red and blue mixed light,blue light and red light have the most significant effects;and blue light and mixed light The content of malondialdehyde has always been at a low level,and the damage to the ginseng leaves is small,which can slow the senescence of the leaves,and the soluble protein and soluble sugar content under these two treatments are the highest.(3)From the growth index,the red and blue mixed light treatment has the best growth.In addition to the red and blue mixed light,compared with the control,yellow light and red light increase the plant height,blue light increases the root thickness,blue light Hehuangguang increases leaf length and width.(4)The content of total saponins is highest under white light,yellow light and red light treatment,the content of PPD type saponin is highest under red light treatment,and the content of PPT type saponin is highest under white light treatment.Red light and yellow light increased the relative expression of DAS gene,and the relative expression of DAS gene was significantly positively correlated with the content of PPD-type saponins and total saponins. |