| Soybean is an important food and oil crop in the world which had-40%protein and-20%oil in its seed.At present,soybean has become the main source of vegetable protein and edible oil for people’s diet.Meanwhile,soybean contains more vitamin E than other crops in seed,and showed an irreplaceable value in many countries.In view of this,one natural population(Pop-1)consisting of 311 soybean varieties and another recombinant inbred line population(Pop-2)consisting of 283 lines were used in this study,and the contents of seed vitamin E(δ-tocopherol,γ-tocopherol,α-tocopherol and total tocopherol),protein and oil were analyzed.The main results are as follows:1.The genetic variations and correlations of seed vitamin E,protein and oil contents of the soybean natural population Pop-1 were studied.The results showed that there existed relative wide genetic variations of the vitamin E,protein and oil contents in the Pop-1 population,and the contents ranged from 159.90 μg/g～382.86 μg/g(total tocopherol),34.68%～44.87%and 16.07%～24.53%,respectively.Meanwhile,the results also showed that there existed significant correlations among vitamin E,protein and oil contents,and the positive correlation between vitamin E and oil,negative correlation between vitamin E and protein contents were detected.2.Twenty-three elite soybean germplasms in Pop-1 population were screened out for its high seed vitamin E,protein and oil contents.Based on the seed vitamin E,protein and oil contents,the natural population was classified into five categories,and the type I and II showed more vitamin E and oil contents than the others.Furthermore,thirteen elite germplasms with high seed vitamin E(Hefeng 50,Hefeng 47 and Henong 75,etc.)and ten elite germplasms with high seed protein or oil contents(Hua-nan-xiao-jin-dou,Ha 13-2958,He-qi,Qian-an-xiao-huang-dou,Hal4-2146,etc.)were selected.3.The genetic variations of vitamin E contents of the soybean recombinant inbred line population Pop-2 were studied,and fourteen elite soybean germplasms were selected for its high seed vitamin E.The results showed that there also existed relative wide genetic variations of the vitamin E in the Pop-2 RIL population,were 14.04%(δ-tocopherol),9.54%(γ-tocopherol),36.59%(α-tocopherol)and 9.24%(total tocopherol),respectively.Based on the seed vitamin E contents,the RIL population was classified into three categories,and the type Ⅰ soybean lines showed more vitamin E contents.Furthermore,fourteen elite RIL lines with high seed vitamin E were selected(L-139,L-325,L-35,L-179 and L-81,etc.).Based on the results,the conclusion are as follows:There existed relative wide genetic variations of the vitamin E,protein and oil contents in the soybean natural population,and the significant correlations among vitamin E,protein and oil contents were detected.Twenty-three elite germplasms with high seed vitamin E,protein and oil contents were selected.The wide genetic variations of vitamin E contents of the soybean recombinant inbred line population were also detected,and fourteen elite RIL lines with high seed vitamin E were also screened out. |
