Transcriptomics Analysis Of Drought Resistance Germination Of Winter Wheat And Preliminary Verification Of Related Genes

Wheat was an important food crop in our country.At present,its high yield mainly depended on measures such as the application of a large amount of chemical fertilizers.However,drought limits the yield and quality of wheat to a certain extent.The root system was the main organ for plants to absorb nutrients.The size of the root system and its plasticity significantly affected the plant’s absorption and utilization efficiency of nutrients.Therefore,this experiment mainly looks for genes with drought resistance during the germination of wheat.In this experiment,different drought-resistant germination wheat varieties Jinmai 47 and Aikang 58 were used as materials to identify genes that respond to drought through transcriptome analysis,and preliminarily verified the response effects of some genes on the germination of wheat drought resistance.The main findings are as follows:Using the indoor germination bag test method,two treatment levels of normal moisture and drought（15%PEG-6000）were set.According to germination rate,longest root length,shoot length,total root length,root surface area,root to shoot ratio,root vitality,phenotypic indicators such as root volume to identify the relationship between drought resistance and germination and the morphological structure of wheat plants.The results showed that after drought stress,the germination rate,longest root length,bud length,total root length,root surface area and root volume of the two varieties at the germination stage were significantly reduced,and the decline of Jinmai 47 was lower than that of Aikang 58.The root-to-shoot ratio and root vigor of the varieties increased,and the increase of Aikang 58 was significantly higher than that of Jinmai 47.It showed that Jinmai 47,which had strong germination resistance under drought conditions,could resist drought stress by maintaining good root morphology.Transcriptome analysis showed that 9096 genes were identified in AK58,of which 3956 were up-regulated and 5140 were down-regulated.3265 genes were identified in JM47,of which 1525 were up-regulated and 1740 were down-regulated.Real-time quantitative PCR verification of abscisic acid related genes was carried out.And analyzing the resulting TRIAE_CS42₄DS_TGACv 1₃61281_AA 1164930 by GO functional analysis and KEGG pathways,TRIAE_CS42₂AL_TGACv1₀94077_AA0292220,TRIAE_CS4 2₂DL_TGACv1₁59916_A A0544370,TRIAE_CS42₄AL_TGACv1₂91776_AA0996560,TRIAE_CS4 2₃B_TGACv 1₂27193_AA0822110,TRIAE_CS42₇DL_TGACv1₆03 500_AA 1984880,TRIA E_CS42₃B_TGACv1₂22632_AA0768310,TRIAE_CS42₃DL_TGA Cv1₂51745_AA088 4440 could cope with stress and involved in a signaling pathway hormone mediated,and could participate in the metabolic process of abscisic acid,and may also regulated the response of wheat root morphology to drought stress.