Analysis Of Case Data On Canine Mammary Tumor And Expression Of SNW1 In Breast Tissue

Objective: The basic data of clinical cases of dog breast tumor in pets were analyzed,the factors related to this disease were summarized,and the potential diagnostic markers and therapeutic targets of dog breast tumor were explored by exploring the expression difference of SNW1 in dog breast tissue and breast tumor.Methods: We collected canine breast tumor cases,summarized the high incidence factors,and identified the histological properties of 16 tissue samples.The differential expression of SNW1 in healthy breast tissues and canine breast tumors with different properties was detected by immunohistochemistry,western blotting and fluorescence quantitative polymerase chain reaction,and the consistency of the expression of SNW1 protein in rodent and canine breast tissues was compared.Results:(1)The results showed that the canine mammary tumors were most common in the last three pairs of mammary glands,and were most common in small dogs and hybrid dogs aged 6-13 years.The incidence of unsterilized dogs was much higher than that of sterilized dogs,accounting for 64%.(2)In 16 cases,the malignant tumors were higher than the benign tumors.The main types of the benign tumors were intraductal papilloma and adenomyoepithelioma,while the malignant tumors were mainly invasive ductal carcinoma and mixed carcinoma.(3)SNW1 protein was all positive in canine breast tumor tissues;The expression of SNW1 in canine breast tumor tissue was slightly higher than that in normal canine breast tissue(p=0.067),showing an upward trend.(4)The SNW1 protein was strongly positive in the breast tumor model mice.Conclusion: The factors of high incidence of breast tumors in dogs are related to the site,breed,and neutering situation,etc.Clinically,the main types of benign tumors are intraductal papilloma and adenomyoepithelioma,while the incidence of invasive ductal carcinoma and mixed carcinoma is high in malignant tumors.It is speculated that the activation of SNW1 can promote the development of breast tumors,and its overexpression is positively correlated with the degree of malignancy of breast tumors,and the expression of this protein is consistent in dogs and rodents.