Effect Of Gut Microbiota On Bioaccessibility Of Two Fungicide By Using SHIME Model

Pesticide residue in vegetables and fruits is the most concerned problem of the quality and safety of agricultural products.Using in vitro simulation method to determine the bioaccessibility of pesticide residues in different fruits and vegetables in gastrointestinal environment after dietary intake,and to evaluate the food safety of pesticide residues in fruits and vegetables has become a research hotspot.Gut microbiota can significantly affect the bioaccessibility of heavy metals and organic pollutants.However,there are few reports on the changes of bioaccessibility of pesticide residues in fruits and vegetables.Based on this,this paper studied prochloraz and prochloraz,which are common in 13 kinds of fruits and vegetables,using the method of SHIME(Simulator of the Human Gut microbiota system of pathogens)in vitro,to study the effect of gut microbiota on the bioaccessibility of pesticides and dietary components in different vegetables and fruits,and to find out the main gut microbiota affecting the bioaccessibility of pesticide residues.The results of this paper will provide a new way to evaluate the health risk of 2 fungicides in fruits and vegetables.1.Determination of prochloraz and Prochloraz bioaccessibility in different fruits and vegetablesThe bioaccessibility of prochloraz and prochloraz in 13 different fruits and vegetables,such as grapes,showed that the bioaccessibility of prochloraz in peanut,soybean and grape were 30.27%,31.36% and 35.61%,respectively,the bioaccessibility of prochloraz in soybean andipuranol was 35.68% and 35.17%,respectively,while the bioaccessibility of prochloraz was 93.02%,94.01% and 97.22% in crab-flavored mushroom,pepper and onion,the bioaccessibility in pepper and maize was 90.01% and 96.84% respectively.2.Effect of gut microbiota on the bioaccessibility of pesticide residues in different fruits and vegetablesTaking grape,ipuranol,soybean and peanut as the representative fruits and vegetables,the effects of intestinal flora on the bioaccessibility of pesticide residues in the fruits and vegetables were studied,the bioaccessibility of prochloraz and prochloraz in grape and soybean decreased significantly with the time of digestion.After 24 hours,the bioaccessibility of prochloraz and prochloraz in grape decreased by 32.21% and 60.27%,respectively,the bioaccessibility of prochloraz and procymidone in soybean was decreased by 46.39% and 44.62% respectively.It can be seen that the intestinal flora is an important factor affecting the bioaccessibility of pollutants.3.The effect of dietary composition on gut microbiota on the bioaccessibility of pesticide residuesThe effects of inulin,resveratrol,protein and vegetable oil on the gut microbiota were studied.The results showed that: 1,inulin enhanced the effect of gut microbiota on the bioaccessibility of pesticides,while resveratrol,protein and vegetable oil had little effect on the bioaccessibility,inulin could reduce the bioaccessibility of prochloraz and prochloraz in grape by 39.55% and 20.83%,respectively.It can be seen that inulin is the most important dietary component affecting the gut microbiota to reduce the bioaccessibility of pesticides in grape.4.The dominant microflora affecting the bioaccessibility of prochloraz and prochloraz in grapesThe study on the effect of two predominant gut microbiota,Escherichia coli and Lactobacillus,on the bioaccessibility of prochloraz and prochloraz in grape indicated that Escherichia coli and Lactobacillus had a time effect on prochloraz,the bioaccessibility of prochloraz in grape was decreased by 33.89% and 31.95% at 36 h,and by 31.19%(Escherichia coli)and 15.22%(Lactobacillus),respectively.Based on the above results,Escherichia coli and Lactobacillus,are the main predominant gut microbiota that affect the bioaccessibility of procymidone.